Abstract: To improve the shelf-life period and the freshness quality of Lychee Fruit, γ-poly (glutamic acid) , a high-molecule biomaterial, was coated on litchi for fresh keeping and stored at normal temperature. The changes of physiological characteristics in storage were studied. The results showed that the treatment of 6g/L γ-PGA could lessen the loss of water content of litchi pericarp, remarkably degrade the respiration rate and the ethylene production rate, slow down the degradation of anthocyanin content, decrease of PPO and POD activities in the pericarp. This method could prolong the fresh-keeping period about 4 days of Lychee Fruit.