HE Shengfa, LONG Caiyun, WANG Jiao, et al. Purification, Identification and Polyclonal Antibody Development for Cow’s Milk Major Allergen αS1-Casein[J]. Science and Technology of Food Industry, 2022, 43(15): 106−114. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110034.
Citation: HE Shengfa, LONG Caiyun, WANG Jiao, et al. Purification, Identification and Polyclonal Antibody Development for Cow’s Milk Major Allergen αS1-Casein[J]. Science and Technology of Food Industry, 2022, 43(15): 106−114. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110034.

Purification, Identification and Polyclonal Antibody Development for Cow’s Milk Major Allergen αS1-Casein

  • Objective: To purify and comprehensive identify αS1-casein from bovine caseins and prepare rabbit polyclonal antibody against αS1-casein. Method: DEAE Sepharose Fast Flow anion exchange chromatography was used to separate and purify αS1-casein. The physicochemical property (isoelectric point and protein content), immunological technique and mass spectrometry were used to comprehensively identify αS1-casein. Then the αS1-casein was obtained by dialysis and lyophilization, polyclonal antibody was prepared by immunizing New Zealand white rabbit with the purified αS1-casein, and the specificity of polyclonal antibody was analyzed. Result: Among the four kinds of caseins, αS1-casein had the latest peak time, the largest peak area in anion exchange chromatography and the highest position in the electrophoretic diagram. Finally, the purity of αS1-casein was 94.26%, and the yield was 27.19%. The titers of sera from two rabbits inoculated with purified αS1-casein for five times reached 1280000 and 320000, respectively. In addition to the slight cross reaction with soybean protein (<0.25%), the antiserum showed no cross reaction with α-Lactalbumin, β-Lactoglobulin, egg white protein and peanut protein, indicating high specificity. Conclusion: This study prepared high purity αS1-casein and high specificity polyclonal antibody against αS1-casein, providing a new train of thought for αS1-casein purification and comprehensive identification, and also providing material basis for the development of immunologic method for detection of αS1-casein.
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