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中国精品科技期刊2020
张敏君,段雪伟,王燕,等. 构树根皮活性成分乙醇提取工艺优化及其抗氧化活性分析[J]. 食品工业科技,2023,44(11):196−203. doi: 10.13386/j.issn1002-0306.2022070304.
引用本文: 张敏君,段雪伟,王燕,等. 构树根皮活性成分乙醇提取工艺优化及其抗氧化活性分析[J]. 食品工业科技,2023,44(11):196−203. doi: 10.13386/j.issn1002-0306.2022070304.
ZHANG Minjun, DUAN Xuewei, WANG Yan, et al. Optimization of Ethanol Extraction Process for Active Components from Broussonetia papyrifera Root Bark and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2023, 44(11): 196−203. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070304.
Citation: ZHANG Minjun, DUAN Xuewei, WANG Yan, et al. Optimization of Ethanol Extraction Process for Active Components from Broussonetia papyrifera Root Bark and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2023, 44(11): 196−203. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070304.

构树根皮活性成分乙醇提取工艺优化及其抗氧化活性分析

Optimization of Ethanol Extraction Process for Active Components from Broussonetia papyrifera Root Bark and Its Antioxidant Activity

  • 摘要: 以构树根皮为原料,通过单因素实验考察不同因素对构树根皮总黄酮和多酚提取量的影响。运用Design-Expert 11软件设计响应面法优化构树根皮乙醇回流提取工艺,并进行工艺验证。最后对提取得到的构树根皮乙醇提取物进行DPPH·、ABTS+·、羟自由基清除能力和总还原能力的测定,评价其抗氧化活性。响应面分析表明,构树根皮总黄酮和多酚的最佳提取工艺为提取温度75 ℃、提取时间117 min、料液比1:16 g/mL、乙醇浓度70%。此条件下,构树根皮总黄酮和多酚提取量分别为23.93±0.30 mg/g和14.69±0.56 mg/g,与预测理论值接近。抗氧化实验表明,构树根皮乙醇提取物对DPPH·、ABTS+·和羟自由基的半数清除浓度(IC50)分别为5.256 μg/mL、0.259 mg/mL和0.310 mg/mL,且清除能力与其浓度呈现一定的量效关系。当提取物浓度为1.0 mg/mL时,总还原能力达到1.484±0.062。此优化实验有效可行,构树根皮乙醇提取物具有较强的抗氧化活性。本研究为构树资源的综合利用提供了一定的理论依据。

     

    Abstract: In this study, single factor experiments were employed to determine the effects of various factors on yields of total flavonoids and polyphenols from Broussonetia papyrifera root bark. Then Box-Behnken design and response surface methodology were used to optimize the ethanol reflux extraction process using the Design-Expert 11 software. Moreover, the antioxidant activity of the ethanol extract of Broussonetia papyrifera root bark was evaluated by determining the scavenging capacity of DPPH·, ABTS+·, and the hydroxyl free radical, as well as the total reducing power. Results showed that the optimal conditions were as follows: Extraction temperature 75 ℃, extraction time 117 min, solid-liquid ratio 1:16 g/mL, and ethanol concentration 70%. Under these conditions, the experimental extraction yield values of total flavonoids and polyphenols from Broussonetia papyrifera root bark were 23.93±0.30 mg/g and 14.69±0.56 mg/g, respectively, which was not significantly different in comparison to predicted values. The IC50 values of scavenging rates on DPPH·, ABTS+·, and the hydroxyl free radical were 5.256 μg/mL, 0.259 mg/mL, and 0.310 mg/mL, respectively, and the scavenging rates showed a certain dose-effect relationship with the sample concentration. In addition, the total reducing power of 1.0 mg/mL ethanol extract of the root bark was 1.484±0.062. These results indicated that this optimization test was effective and feasible, and the ethanol extract of Broussonetia papyrifera root bark had good antioxidant activity in vitro. The present study provides supplement information for the comprehensive utilization of Broussonetia papyrifera in food and medicine ingredients.

     

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