蜂王浆中脂肪酸标志物检测方法的研究进展

于心雨; 李珊珊; 陶凌晨; 张国志; 胡福良

doi:10.13386/j.issn1002-0306.2022040049

蜂王浆中脂肪酸标志物检测方法的研究进展

doi: 10.13386/j.issn1002-0306.2022040049

浙江大学动物科学学院，浙江省蚕蜂资源利用与创新研究重点实验室，浙江杭州 310058

基金项目: 国家自然科学基金（31872431）；国家蜂产业技术体系专项（CARS-44）；浙江省“三农九方”科技协作计划项目（2022SNJF056）。

详细信息

作者简介:
于心雨（1998−），女，硕士研究生，研究方向：蜂产品及功能性食品科学，E-mail：yuxinyu@zju.edu.cn

通讯作者:
胡福良（1964−），男，博士，教授，研究方向：蜜蜂、蜂产品及功能性食品科学，E-mail：flhu@zju.edu.cn

中图分类号: S896.3
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- 文章访问数: 44
- HTML全文浏览量: 15
- PDF下载量: 5
- 被引次数: 0
出版历程
- 收稿日期: 2022-04-07
- 网络出版日期: 2022-12-16
- 刊出日期: 2023-01-17

Recent Advances in Determination Methods of Fatty Acid Markers in Royal Jelly

Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

摘要

摘要: 蜂王浆是青年工蜂咽下腺和上颚腺分泌的乳白色、淡黄色或浅橙色浆状物质，脂肪酸是蜂王浆的主要活性成分之一，主要包括反式-10-羟基-2-癸烯酸、10-羟基癸酸、3,10-二羟基-癸酸、8-羟基辛酸、癸二酸等，具有抗炎、抗菌和抗肿瘤等多种生物活性。在国家标准中，蜂王浆脂肪酸是评价蜂王浆质量的重要指标，因此脂肪酸的检测对蜂王浆的质量控制具有十分重要的作用。本文综述了目前蜂王浆脂肪酸的检测方法，简要介绍了检测方法的基本原理，同时对不同检测技术和应用特点进行比较，并对今后检测发展的趋势进行了探讨，为进一步开发高效的脂肪酸检测方法及蜂王浆的质量控制提供一定的理论参考。
- 蜂王浆 /
- 脂肪酸 /
- 检测方法 /
- 质量控制 /
- 反式-10-羟基-2-癸烯酸
Abstract: Royal jelly is a yellowish, creamy and acidic substance secreted by mandibular and hypopharyngeal glands of young worker bees. Fatty acids including (E)-10-hydroxy-2-decenoic acid, 10-hydroxydecanoic acid, 3,10-dihydroxydecanoic acid, 8-hydroxyoctanoic acid, sebacic acid and so on are one of the major components of royal jelly with a variety of biological activities, such as anti-inflammation, anti-bacteria and anti-tumor. Fatty acids are one of the main active components of royal jelly which are considered an important indicator in quality control. This article summarizes the identification methods of the royal jelly fatty acid including the principle of the method, the comparisons of technology and application features of methods, as well as the future research prospects. This review will provide a reference for the development of determining methods for fatty acids and pave the way for quality control of royal jelly.
- royal jelly /
- fatty acids /
- determination methods /
- quality control /
- (E)-10-hydroxy-2-decenoic acid

HTML全文

图 1 蜂王浆中主要脂肪酸的分子结构图

Figure 1. Structures of main fatty acids in royal jelly

下载: 全尺寸图片幻灯片

图 2 10-HDA的生物活性及作用途径示意图

Figure 2. Schematic illustration of the biological activities and pathways of 10-HDA

注：生物活性示意图由BioRender.com创建。

下载: 全尺寸图片幻灯片

表 1 蜂王浆脂肪酸检测方法及脂肪酸种类

Table 1. Determination methods and types of fatty acids of royal jelly

蜂王浆种类	检测方法	脂肪酸种类	参考文献
新鲜蜂王浆	HPLC	10-HDA	Bloodworth等^[32]
蜂王浆制剂（冻干粉、颗粒剂、口服液）	HPLC	10-HDA	杨江丰等^[37]
新鲜蜂王浆	HPLC	10-HDA	张娟等^[38]
新鲜蜂王浆	HPLC	10-HDA	罗小凤等^[39]
新鲜蜂王浆和蜂王浆制剂	HPLC	10-HDA	Genc等^[34]
蜂王浆冻干粉、蜂蜜、含有蜂王浆的蜂蜜	HPLC	10-HDA	Santos等^[40]
新鲜蜂王浆、蜂王浆冻干粉	超高效液相色谱法、HPLC	10-HDA	Zhou等^[41]
新鲜蜂王浆	超高效液相色谱-串联质谱法	癸二酸	李兴安等^[42]
新鲜蜂王浆	高效液相色谱-质谱法	10-HDA、8-羟基辛酸、癸二酸、10-羟基癸酸、 2-十二碳烯二酸和十二烷基二酸	刘善菁等^[43]
新鲜蜂王浆	液相色谱-高分辨质谱法	10-HDA、10-羟基癸酸、3-羟基癸酸、癸二酸和2-十二烯二酸	Kokotou等^[16]
新鲜蜂王浆	气相色谱-质谱联用法	28种脂肪酸（10-HDA、10-羟基癸酸、癸二酸等）	Lercker等^[49]
蜂王浆冻干粉	气相色谱-质谱联用法	35种脂肪酸（10-HDA、10-羟基癸酸、癸二酸等）	Isidorov等^[50]
新鲜蜂王浆	顶空固相微萃取-气相色谱-质谱联用法	85种脂肪酸（10-HDA、10-羟基癸酸、癸二酸等）	Isidorov等^[51]
新鲜蜂王浆	毛细管气相色谱法	14种脂肪酸（10-HDA、10-羟基癸酸、癸二酸等）	Ferioli等^[52]
蜂王浆制剂	气相色谱法	10-HDA	李小佳等^[57]
新鲜蜂王浆	毛细管电泳法、HPLC	10-HDA	Ferioli等^[60]
新鲜蜂王浆	毛细管电泳法	10-HDA	Munoz等^[61]
蜂王浆类膳食补充剂	特制毛细管电泳-非接触电导检测法	10-HDA	Duong等^[63]
新鲜蜂王浆	近红外光谱结合化学计量学法	10-HDA	Yang等^[66]
新鲜蜂王浆	ATR-FTMIR与近红外光谱结合化学计量学法	10-HDA	Yang等^[67]
新鲜蜂王浆、蜂王浆冻干粉	酶联免疫吸附检测法	10-HDA	Kaljurand等^[58]
新鲜蜂王浆	紫外分光光度法	10-HDA	赵静^[70]
新鲜蜂王浆	比色法	10-HDA	Su等^[10]

下载: 导出CSV

表 2 不同蜂王浆脂肪酸检测方法的比较

Table 2. Comparison of different determination methods of fatty acids in royal jelly

检测方法	优势	劣势
高效液相色谱法	检测范围广、检出率高、重复性好	设备成本较高、检测时间较长、试剂消耗量大
高效液相色谱-质谱联用法	灵敏度高、选择性高、分离能力好	仪器复杂、价格昂贵、试剂消耗量大
超高效液相色谱法	准确度高、检测时间短、检测效率高	设备成本较高、操作步骤复杂、对样品前处理要求高
气相色谱-质谱联用法	有毒试剂消耗量小、灵敏度高、准确性好	需衍生化、操作复杂、设备成本高
毛细管电泳法	操作简便、分离效率高、溶剂消耗少、成本低	精确性较差、重复性较差、对样品纯度要求较高
近红外光谱法	快速、无损、无需消耗化学试剂	需结合统计学方法建立分析模型
酶联免疫吸附剂检测法	检测成本低、效率高、操作简便	应用范围小、准确度低、定量效果差
紫外分光光度法	设备成本较低、操作便捷、分析速度快	应用范围小、灵敏度低、对样品纯度要求较高
比色传感器检测法	分析速度快、可直接观测检测效果、可用手机进行读数定量	应用范围小、特异性较强

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