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中国精品科技期刊2020

油橄榄叶多糖的提取工艺优化及其理化性质和抗氧化活性

任一杰 赵小亮 王宝忠 向紫骏 杨超福 王海利 马君义 张伟杰

任一杰,赵小亮,王宝忠,等. 油橄榄叶多糖的提取工艺优化及其理化性质和抗氧化活性[J]. 食品工业科技,2022,43(23):245−251. doi:  10.13386/j.issn1002-0306.2022030302
引用本文: 任一杰,赵小亮,王宝忠,等. 油橄榄叶多糖的提取工艺优化及其理化性质和抗氧化活性[J]. 食品工业科技,2022,43(23):245−251. doi:  10.13386/j.issn1002-0306.2022030302
REN Yijie, ZHAO Xiaoliang, WANG Baozhong, et al. Optimization of Extraction Process of Polysaccharides from Olea europaea L. Leaves and Its Physicochemical Properties and Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(23): 245−251. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022030302
Citation: REN Yijie, ZHAO Xiaoliang, WANG Baozhong, et al. Optimization of Extraction Process of Polysaccharides from Olea europaea L. Leaves and Its Physicochemical Properties and Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(23): 245−251. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022030302

油橄榄叶多糖的提取工艺优化及其理化性质和抗氧化活性

doi: 10.13386/j.issn1002-0306.2022030302
基金项目: 国家自然科学基金(31860250,32260231);甘肃省自然科学基金(18JR3RA148);甘肃省基础研究创新群体计划项目(1506RJIA116);甘肃省科技创新专项基金项目(2019ZX-05)。
详细信息
    作者简介:

    任一杰(1981−),男,本科,高级工程师,研究方向:天然产物化学,E-mail:471208356@qq.com

    通讯作者:

    赵小亮(1982−),男,博士,副教授,研究方向:糖化学与糖药物,E-mail:zhxl819@163.com

    张伟杰(1975−),男,博士,教授,研究方向:糖基医用生物材料,E-mail:brossica@163.com

  • 中图分类号: TS201.1

Optimization of Extraction Process of Polysaccharides from Olea europaea L. Leaves and Its Physicochemical Properties and Antioxidant Activity

  • 摘要: 为增加油橄榄叶多糖的开发利用价值,以油橄榄叶为实验材料,采用正交试验优化油橄榄叶多糖(OLP)的提取工艺,高效凝胶渗透色谱-多角度激光光散射仪联用(HPGPC-MALLS)测定分子量,PMP柱前衍生高效液相色谱法分析OLP的单糖组成,并评价其抗氧化活性。结果显示,OLP的最佳提取工艺条件为料液比1:27.5 g/mL,温度95 ℃,提取时间3.5 h,在此条件下OLP的得率为2.75%;OLP的重均分子量(Mw)为25.36 kDa,数均分子量(Mn)为19.32 kDa,多分散系数为1.313;OLP主要由葡萄糖(Glc)、半乳糖(Gal)和氨基半乳糖(GalN)组成,还含有鼠李糖(Rha)、阿拉伯糖(Ara)、木糖(Xyl)、甘露糖(Man)和氨基葡萄糖(GlcN)等单糖,各单糖的相对摩尔比为56.2:15.9:10.3:8.3:5.9:2.6:0.5:0.3;抗氧化活性实验结果发现,OLP具有较好的抗氧化活性,对羟自由基、超氧阴离子自由基和DPPH自由基的半数抑制浓度(IC50)分别为0.422、0.302和0.268 mg/mL。本研究所得油橄榄叶多糖的提取工艺简单、得率高、抗氧化活性好,为油橄榄叶多糖的进一步研究和开发利用提供了重要参考。
  • 图  1  料液比对多糖得率的影响

    Figure  1.  Effects of material-liquid ratio on the yield of polysaccharides

    注:不同小写字母表示差异显著(P<0.05);图2~图3同。

    图  2  温度对多糖得率的影响

    Figure  2.  Effects of temperature on the yield of polysaccharides

    图  3  时间对多糖得率的影响

    Figure  3.  Effects of time on the yield of polysaccharides

    图  4  OLP红外光谱图

    Figure  4.  Infrared spectrogram of OLP

    图  5  OLP单糖组成分析HPLC图

    Figure  5.  Monosaccharide composition analysis of OLP by HPLC

    注:1. Man;2. GlcN;3. Rha;4. GlcA;5. GalN;6. GalA;7. Glc;8. Gal;9. Xyl;10. Ara。

    图  6  OLP对羟自由基的清除活性

    Figure  6.  Scavenging activity of OLP on hydroxyl free radical

    图  7  OLP对超氧阴离子自由基的清除活性

    Figure  7.  Scavenging activity of OLP on superoxide anion free radical

    图  8  OLP对DPPH自由基的清除活性

    Figure  8.  Scavenging activity of OLP on DPPH free radical

    表  1  正交试验因素水平

    Table  1.   Orthogonal assay factors and levels

    水平因素
    A 料液比
    (g/mL)
    B 温度
    (℃)
    C 时间
    (h)
    11:27.5852.5
    21:30.0903.0
    31:32.5953.5
    下载: 导出CSV

    表  2  L9(34)正交实验及结果

    Table  2.   L9(34) orthogonal design and experiment results

    序号影响因素多糖得
    率(%)
    A 料液比B 温度C 时间D (空白列)
    111112.10
    212222.51
    313332.74
    421232.07
    522312.42
    623122.24
    731322.19
    832132.44
    933212.46
    K17.356.366.786.98
    K26.737.377.046.94
    K37.097.447.357.25
    k12.452.122.262.33
    k22.242.462.352.31
    k32.362.482.452.42
    R0.210.360.190.11
    下载: 导出CSV

    表  3  OLP分子量分析结果

    Table  3.   OLP molecular weight analysis results

    OLPMw(kDa)Mn(kDa)多分散系数(Mw/Mn)
    25.3619.321.313
    下载: 导出CSV

    表  4  OLP单糖组成及相对摩尔比分析结果

    Table  4.   The analysis results of monosaccharide composition and relative mole ratio

    样品单糖组成及其相对摩尔比
    ManGlcNRhaGalNGlcGalXylAra
    OLP0.50.38.310.356.215.92.65.9
    下载: 导出CSV
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  • 收稿日期:  2022-03-24
  • 网络出版日期:  2022-10-21
  • 刊出日期:  2022-11-23

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