松茸多糖的体外抗氧化性及对乙醇氧化损伤小鼠作用的研究

沈成龙; 陈怡帆; 曾英杰; 陈炼红

doi:10.13386/j.issn1002-0306.2022020248

松茸多糖的体外抗氧化性及对乙醇氧化损伤小鼠作用的研究

doi: 10.13386/j.issn1002-0306.2022020248

西南民族大学食品科学与技术学院，四川成都 610041

基金项目: 西南民族大学中央高校基本科研业务费专项资金项目资助（2022NYXXS013）。

详细信息

作者简介:
沈成龙（2000−），男，硕士研究生，研究方向：食品加工与安全，E-mail：1402289402@qq.com

通讯作者:
陈炼红（1967−），女，硕士，教授，研究方向：民族食品资源开发与研究，E-mail：lianhong_chen@163.com

中图分类号: TS201.4
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- 被引次数: 0
出版历程
- 收稿日期: 2022-02-28
- 网络出版日期: 2022-10-21
- 刊出日期: 2022-11-23

Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation

College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China

摘要

摘要: 本文以超氧阴离子自由基（Superoxide anion radicals，O₂^－ · ）、2，2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐（2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)，ABTS）自由基清除能力、总还原能力为指标研究松茸多糖（Tricholoma matsutake polysaccharide，TMP）体外抗氧化能力；建立乙醇氧化损伤小鼠模型，通过测定小鼠血清中谷丙转氨酶（Alanine transaminase，ALT）、谷草转氨酶（Aspartate transaminase，AST）和碱性磷酸酶（Alkaline phosphatase，AKP）的活力以及肝脏中过氧化氢酶（Catalase，CAT）、丙二醛（Malondialdehyde，MDA）、超氧化物歧化酶（Superoxide dismutase，SOD）和谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-Px）的水平研究松茸多糖的体内抗氧化活性。体外抗氧化结果表明，松茸多糖对超氧阴离子自由基和ABTS自由基有较好的清除能力，当浓度为1 mg/mL时，清除率分别为56.67%和96.60%，而松茸多糖的总还原能力较低，当浓度为1 mg/mL，吸光值为0.163；动物实验结果表明，连续灌胃四周后，与模型对照组相比，松茸多糖可以使小鼠血清中的ALT、AST和AKP活力显著降低（P<0.05），使肝脏中CAT、SOD和GSH-Px的水平显著升高（P<0.05），MDA的含量显著降低（P<0.05），且呈剂量依赖性。研究表明松茸多糖有一定的抗氧化活性，可为松茸多糖的后续开发应用提供思路。
- 松茸多糖 /
- 体外抗氧化 /
- 体内抗氧化 /
- 乙醇氧化损伤
Abstract: In order to study the antioxidant activity of Tricholoma matsutake polysaccharides (TMP), its antioxidant capacity in vitro was investigated using superoxide anion radical (O₂^－ · ) scavenging capacity, total reducing capacity and ABTS radical scavenging capacity as indicators; and then the mouse model of ethanol oxidative damage was established, and the antioxidant activity of Tricholoma matsutake polysaccharides in vivo were measured by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) in the serum of mice and the activity of catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver. In vitro antioxidant results showed that Tricholoma matsutake polysaccharides had better scavenging ability on superoxide anion free radical and ABTS free radical, and the scavenging rate reached 56.67% and 96.60% when the concentration was 1 mg/mL. However, the total reducing ability of Tricholoma matsutake polysaccharides was not obvious. When the concentration was 1 mg/mL, the absorbance value could reach 0.163. Animal experiment results showed that after four weeks of continuous gastric administration, compared with the model control group, Tricholoma matsutake polysaccharides could significantly reduce ALT, AST and AKP viability in mice serum (P<0.05); it also significantly increased the levels of CAT, SOD and GSH-Px in liver (P<0.05), when the content of MDA was significantly reduced (P<0.05). All these indicators showed a dose-response relationship. The experimental results showed that Tricholoma matsutake polysaccharides had certain antioxidant activity, hoping to provide ideas for the subsequent development and application of Tricholoma matsutake polysaccharides.
- Tricholoma matsutake polysaccharide /
- in vitro antioxidant /
- in vivo antioxidant /
- ethanol oxidation damage

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图 1 松茸多糖对超氧阴离子自由基的清除能力

Figure 1. The scavenging ability of Tricholoma matsutake polysaccharides on superoxide anion free radicals

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图 2 松茸多糖的总还原能力

Figure 2. The total reducing power of Tricholoma matsutake polysaccharides

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图 3 松茸多糖对ABTS自由基的清除能力

Figure 3. The ability of Tricholoma matsutake polysaccharides to scavenge ABTS free radicals

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图 4 小鼠肝脏切片（400×）

Figure 4. Mouse liver section（400×）

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表 1 实验小鼠分组及处理

Table 1. Grouping and treatment of experimental mice

组别	灌胃试剂	灌胃剂量
空白对照组	生理盐水	0.3 mL
模型对照组	生理盐水	0.3 mL
阳性对照组	V_C	100 mg/kg
低剂量组	松茸多糖溶液	100 mg/kg
中剂量组	松茸多糖溶液	200 mg/kg
高剂量组	松茸多糖溶液	400 mg/kg

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表 2 小鼠体重变化

Table 2. Changes in body weight of mice

组别	小鼠体重（g）
组别	0 d	7 d	14 d	21 d	28 d
空白对照组	30.4±0.32	31.9±0.42	33.7±0.51	34.8±0.37	36.0±0.22
模型对照组	29.8±0.55	32.3±0.62	33.1±0.35	34.3±0.44	35.7±0.29
阳性对照组	30.2±0.45	32.1±0.58	33.4±0.61	34.9±0.45	35.8±0.71
低剂量组	30.3±0.37	32.3±0.32	33.8±0.29	34.9±0.54	35.6±0.27
中剂量组	30.5±0.28	31.8±0.33	33.5±0.56	35.0±0.27	36.2±0.61
高剂量组	30.1±0.43	32.5±0.51	33.6±0.36	34.9±0.41	36.1±0.52

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表 3 松茸多糖对小鼠血清中ALT、AST、AKP活力的影响

Table 3. Effect of Tricholoma matsutake polysaccharides on the activities of ALT, AST and AKP in the serum of mice

组别	剂量（mg/kg）	ALT (U/L)	AST （U/L）	AKP （U/L）
空白对照组	0	14.61±0.59	32.43±1.37	56.26±3.28
模型对照组	0	28.33±1.29^#	60.19±1.72^#	182.28±9.42^#
阳性对照组	100	17.76±1.02^*	37.22±1.02^*	75.89±4.78^*
低剂量组	100	24.56±1.06^*&	45.64±1.27^*&	147.65±11.92^*&
中剂量组	200	22.34±1.39^*&	42.96±1.43^*&	136.02±10.21^*&
高剂量组	400	20.16±1.03^*	40.22±1.04^*	109.88±6.28^*&
注：#：与空白对照组比较，差异显著（P<0.05）； *：与模型对照组比较，差异显著（P<0.05）； &：与阳性对照组比，差异显著（P<0.05）；表4同。

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表 4 松茸多糖对小鼠肝脏中CAT、MDA、SOD和GSH-Px水平的影响

Table 4. Effects of Tricholoma matsutake polysaccharides on the levels of CAT, MDA, SOD and GSH-Px in the liver of mice

组别	剂量 (mg/kg)	CAT (U/mg prot)	SOD (U/mg prot)	MDA (nmol/mg prot)	GSH-Px (U/mg prot)
空白对照组	0	97.03±2.84	152.83±2.88	4.60±0.38	78.60±3.83
模型对照组	0	44.41±1.63^#	110.58±2.17^#	10.61±0.27^#	41.67±1.23^#
阳性对照组	100	89.05±3.24^*	150.88±2.65^*	5.23±0.22^*	74.46±2.98^*
低剂量组	100	56.73±1.67^*&	117.44±1.89^&	8.30±0.66^*&	48.55±1.09^&
中剂量组	200	62.45±2.21^*&	120.89±1.22^*&	8.16±0.94^*&	47.93±1.94^&
高剂量组	400	72.42±1.78^*&	144.52±2.51^*	6.29±0.44^*	68.47±2.06^*

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