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中国精品科技期刊2020
姚沛琳,刘梦茹,杨澳,等. 基于非靶向代谢组学的蓝莓酵素和沙棘酵素代谢产物特征比较[J]. 食品工业科技,2022,43(19):160−166. doi: 10.13386/j.issn1002-0306.2021120282.
引用本文: 姚沛琳,刘梦茹,杨澳,等. 基于非靶向代谢组学的蓝莓酵素和沙棘酵素代谢产物特征比较[J]. 食品工业科技,2022,43(19):160−166. doi: 10.13386/j.issn1002-0306.2021120282.
YAO Peilin, LIU Mengru, YANG Ao, et al. Comparison of Metabolite Characteristics of Blueberry Jiaosu and Sea-buckthorn Jiaosu Based on Non-targeted Metabolomics Approach[J]. Science and Technology of Food Industry, 2022, 43(19): 160−166. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120282.
Citation: YAO Peilin, LIU Mengru, YANG Ao, et al. Comparison of Metabolite Characteristics of Blueberry Jiaosu and Sea-buckthorn Jiaosu Based on Non-targeted Metabolomics Approach[J]. Science and Technology of Food Industry, 2022, 43(19): 160−166. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120282.

基于非靶向代谢组学的蓝莓酵素和沙棘酵素代谢产物特征比较

Comparison of Metabolite Characteristics of Blueberry Jiaosu and Sea-buckthorn Jiaosu Based on Non-targeted Metabolomics Approach

  • 摘要: 为了探索不同酵素之间代谢产物的差异性,本研究以自然发酵的蓝莓酵素和沙棘酵素为样品,使用超高效液相色谱-四级杆飞行时间质谱测定两种酵素样品中全部代谢物丰度,并对代谢物进行筛选和鉴定,利用代谢组学方法分析蓝莓酵素和沙棘酵素的代谢产物差异。结果表明,两种酵素样品中关键性代谢差异物为9种,通过KEGG数据库的比对分析可知富集代谢产物较多且显著性高的关键代谢通路有6条,主要体现在黄酮和黄酮醇的生物合成,泛酸和辅酶A的生物合成,酪氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,脯氨酸和精氨酸代谢,谷胱甘肽代谢。基于非靶向代谢组学分析,该文初步揭示了蓝莓和沙棘酵素代谢产物差异性,为水果酵素功能性的比较研究提供一定的理论基础和依据。

     

    Abstract: In order to explore the differences of metabolites between different Jiaosu samples, ultra-high performance liquid chromatography coupled to quadrupole time of flight mass spectrometry was used to identify and analyze the differential metabolites of blueberry jiaosu and sea-buckthorn jiaosu with metabolomics method. The results showed that 9 key metabolic differences in the two jiaosu samples. The comparative analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database indicated that the high metabolites enrichment and high significance involved six metabolic pathways, which were flavonoid and flavonol biosynthesis, pantothenic acid and coenzyme A biosynthesis, tyrosine metabolism, alanine, aspartic acid and glutamate metabolism, proline and arginine metabolism, and glutathione metabolism. According to the non-targeted metabolome analysis, this research initially revealed the differences in the metabolites of blueberry jiaosu and sea-buckthorn jiaosu. Accordingly, a theoretical reference has been presented for the study of function of fruit jiaosu.

     

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