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中国精品科技期刊2020
张妹,张彦青,解军波,等. 6′′′-阿魏酰斯皮诺素对Aβ1-42诱导损伤的SH-SY5Y细胞保护作用研究[J]. 食品工业科技,2022,43(13):373−380. doi: 10.13386/j.issn1002-0306.2021120012.
引用本文: 张妹,张彦青,解军波,等. 6′′′-阿魏酰斯皮诺素对Aβ1-42诱导损伤的SH-SY5Y细胞保护作用研究[J]. 食品工业科技,2022,43(13):373−380. doi: 10.13386/j.issn1002-0306.2021120012.
ZHANG Mei, ZHANG Yanqing, XIE Junbo, et al. Protective Effect of 6′′′-Feruloylspinosin on Aβ1-42-Induced SH-SY5Y Cells Injury[J]. Science and Technology of Food Industry, 2022, 43(13): 373−380. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120012.
Citation: ZHANG Mei, ZHANG Yanqing, XIE Junbo, et al. Protective Effect of 6′′′-Feruloylspinosin on Aβ1-42-Induced SH-SY5Y Cells Injury[J]. Science and Technology of Food Industry, 2022, 43(13): 373−380. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120012.

6′′′-阿魏酰斯皮诺素对Aβ1-42诱导损伤的SH-SY5Y细胞保护作用研究

Protective Effect of 6′′′-Feruloylspinosin on Aβ1-42-Induced SH-SY5Y Cells Injury

  • 摘要: 目的:探讨酸枣仁黄酮类成分6′′′-阿魏酰斯皮诺素预处理对Aβ1-42诱导损伤SH-SY5Y细胞的保护作用。方法:利用6′′′-阿魏酰斯皮诺素(1、5、10、20和40 μmol/L)预处理SH-SY5Y细胞2 h,随后加入5 μmol/L的Aβ1-42共同孵育24 h,利用CCK-8法检测细胞活力;Calcein-AM/PI双染法观察细胞形态;试剂盒检测细胞内活性氧、丙二醛、线粒体膜电位水平及谷胱甘肽过氧化物酶活力;蛋白免疫印迹法检测细胞凋亡相关蛋白:Bcl-2关联死亡启动子重组蛋白、B细胞淋巴瘤/白血病-2蛋白的表达。结果:6′′′-阿魏酰斯皮诺素可以抑制Aβ1-42诱导的细胞凋亡,提高细胞活力(P<0.001);改善Aβ1-42诱导损伤细胞的氧化应激情况,降低细胞内活性氧(P<0.001)、丙二醛水平(P<0.01),提高谷胱甘肽过氧化物酶活力(P<0.001);改善Aβ1-42诱导损伤的细胞线粒体功能,上调细胞线粒体膜电位水平(P<0.001);增加抗凋亡蛋白B细胞淋巴瘤/白血病-2蛋白的表达(P<0.01),并降低促凋亡蛋白Bcl-2关联死亡启动子重组蛋白的表达(P<0.01)。结论:6′′′-阿魏酰斯皮诺素对Aβ1-42诱导损伤的SH-SY5Y细胞具有保护作用,其机制与6′′′-阿魏酰斯皮诺素抗氧化活性及调节凋亡相关蛋白表达有关。

     

    Abstract: Objective: To investigate the protective effects of 6′′′-feruloylspinosinon Aβ1-42-induced SH-SY5Y cells injury. Methods: SH-SY5Y cells were pretreated with 6′′′-feruloylspinosin (1、5、10、20 and 40 μmol/L) for 2 h and then co-treated with 5 μmol/L of Aβ1-42 for 24 h. Cell viability was measured by CCK-8 assay; cell morphology was observed by Calcein-AM/PI assay; intracellular reactive oxygen species, malonic dialdehyde level, Glutathione peroxidaseactivity and mitochondrial membrane potential level were detected by the kit; expression of apoptosis-related proteins: Bcl-xL/Bcl-2 associated death promoter, B-cell lymphoma/leukemia-2 were detected by Western Blotting. Results: 6′′′-feruloylspinosin could inhibit Aβ1-42-induced apoptosis and improve cell viability (P<0.001); reduce intracellular reactive oxygen species (P<0.001) and malondialdehyde (P<0.01) levels; increase glutathione peroxidase activity (P<0.001); upregulate cell mitochondrial membrane potential level (P<0.001); regulate apoptosis-related protein expression (P<0.01). Conclusion: The results validated that 6′′′-feruloylspinosin had a protective effect on SH-SY5Y cells with Aβ1-42-induced damage, and the mechanism was that 6′′′-feruloylspinosin could alleviate oxidative damage and regulate the expression of apoptosis related proteins.

     

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