不溶性豆渣纤维对豆渣蛋白乳液特性的影响

王中江; 郭亚男; 刘双奇; 李柏良; 张震; 刘军; 冯镇; 郭增旺

doi:10.13386/j.issn1002-0306.2021080302

不溶性豆渣纤维对豆渣蛋白乳液特性的影响

doi: 10.13386/j.issn1002-0306.2021080302

1.
东北农业大学食品学院，黑龙江哈尔滨 150030
2.
山东禹王生态食业有限公司，山东德州 251200

基金项目: 黑龙江省普通本科高等学校青年创新人才培养计划（UNPYSCT-2018163）；青年人才托举工程项目（2019QNRC001）；山东省重点研发计划（重大科技创新项目）（2022CXGC010603）；黑龙江省“百千万”工程科技重大专项项目（2021ZX12B02）；“十四五”国家重点研发计划（2021YFD2100400）；黑龙江省重大科技成果转化项目（CG19A002）；山东省新旧动能转换重大产业攻关项目。

详细信息

作者简介:
王中江（1987−），男，博士，副教授，研究方向：粮食、油脂及植物蛋白工程，E-mail：wzjname@126.com

通讯作者:
郭增旺（1992−），男，博士，副教授，研究方向：粮食、油脂及植物蛋白工程，E-mail：gzwname@163.com

中图分类号: TS214.2
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- 文章访问数: 56
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- 被引次数: 0
出版历程
- 收稿日期: 2021-08-27
- 网络出版日期: 2022-12-14
- 刊出日期: 2023-01-17

Effect of Insoluble Okara Fiber on the Properties of Okara Protein Emulsion

1.
College of Food Science, Northeast Agricultural University, Harbin 150030, China
2.
Shandong Yuwang Ecological Food Industry Co., Ltd., Dezhou 251200, China

摘要

摘要: 本文探究不溶性豆渣纤维（IOF）对豆渣蛋白（SOP）乳液性能的影响。以葵花籽油为油相，研究不同浓度的IOF（0.25 wt%、0.50 wt%、0.75 wt%和1.00 wt%）对SOP乳液微观结构、界面活性、乳化性和稳定性的影响。结果表明：随着IOF浓度的升高，SOP/IOF乳液的粒径呈现逐渐升高的趋势，激光共聚焦表明乳液液滴变化呈现相同趋势，ζ-电位绝对值呈现升高趋势，界面蛋白含量和浓度呈现增大趋势，流变指标的变化表明剪切稀化的趋势在加强，且高浓度的IOF能显著增强SOP乳液的长期储存稳定性、耐酸稳定性及耐盐稳定性，这为IOF在蛋白乳液凝胶体系中的进一步应用提供了理论基础。
- 不溶性豆渣纤维 /
- 豆渣蛋白 /
- 乳化特性 /
- 乳液稳定性
Abstract: This article explored the effect of insoluble okara fiber (IOF) on the properties of okara protein (SOP) emulsion. The effects of different concentrations of IOF (0.25 wt%, 0.50 wt%, 0.75 wt% and 1.00 wt%) on the microstructure, interfacial activity, emulsifying activity and stability of SOP emulsion were studied with sunflower oil as oil phase. The results showed that with the increase of the IOF concentration, the particle size of the SOP/IOF emulsion showed a trend of gradual increase. The laser confocal microscope observation experiment showed that the emulsion droplets change in the same trend, and the absolute value of the zeta potential presents an increasing trend. The content and concentration of interfacial protein showed an increasing trend, and the change of rheological index showed that the trend of shear thinning was increasing, and high concentration of IOF could significantly enhance the long-term storage stability, acid resistance stability and salt resistance stability of SOP emulsion. This provides a theoretical basis for the further application of IOF in the protein emulsion gel system.
- insoluble okara fiber /
- okara protein /
- emulsifying properties /
- emulsion stability

HTML全文

图 1 IOF对SOP乳液微观结构的影响

Figure 1. The effect of IOF concentration on the microstructure of SOP emulsion

注：IOF添加量：A：0；B：0.25 wt%；C：0.50 wt%；D：0.75 wt%；E：1.00 wt%。

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图 2 IOF浓度对SOP乳液界面张力的影响

Figure 2. The effect of different concentrations of IOF on the interfacial tension of SOP emulsion

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图 3 IOF浓度对SOP/IOF复合乳液的表观粘度的影响

Figure 3. The effect of different concentrations of IOF on the apparent viscosity of SOP/IOF composite emulsion

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图 4 IOF浓度对SOP/IOF复合乳液G′的影响

Figure 4. The effect of different concentrations of IOF on the storage modulus of SOP/IOF composite emulsion

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图 5 IOF浓度对SOP/IOF复合乳液G′′的影响

Figure 5. The effect of different concentrations of IOF on the loss modulus of SOP/IOF composite emulsion

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图 6 IOF浓度对SOP/IOF复合乳液 tanδ的影响

Figure 6. The effect of different concentrations of IOF on the loss tangent of SOP/IOF composite emulsion

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图 7 IOF浓度对SOP/IOF复合乳液乳化性的影响

Figure 7. The effect of different concentrations of IOF on emulsifying activity of SOP/IOF composite emulsion

注：不同小写字母表示差异显著（P<0.05）。

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表 1 IOF浓度对SOP乳液粒径和ζ-电位的影响

Table 1. The effect of IOF concentration on the particle size and ζ-potential of SOP emulsion

IOF浓度（wt%）	ζ-电位（mV）	乳液平均粒径（μm）
0	−35.2±0.3^e	4.81±0.19^e
0.25	−40.2±0.2^d	6.34±0.21^d
0.50	−42.0±0.2^c	13.98±0.45^c
0.75	−43.3±0.3^b	18.49±0.72^b
1.00	−43.9±0.2^a	26.09±0.82^a
注：同列不同小写字母表示差异显著（P<0.05），表2、表3同。

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表 2 IOF浓度对SOP乳液界面蛋白含量和浓度的影响

Table 2. The effect of IOF concentration on the protein content and concentration of SOP emulsion interface

IOF浓度（wt%）	界面蛋白含量（P_A，%）	界面蛋白浓度（Γ，mg/m²）
0	34.211±0.106^d	0.127±0.002^c
0.25	38.153±0.062^c	0.131±0.003^c
0.50	41.785±0.099^b	0.169±0.003^a
0.75	42.062±0.150^b	0.174±0.004^a
1.00	46.566±0.271^a	0.151±0.009^b

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表 3 IOF浓度对SOP/IOF复合乳液的流动行为的影响

Table 3. The effect of different concentrations of IOF on the flow behavior of SOP/IOF composite emulsion

IOF浓度（wt%）	初始表观黏度（Pa·s）	稠度指数（Pa.sn）	流动行为指数	R²
0	3.681±0.940^d	2.734±0.619^c	0.9784±0.0163^a	0.9989
0.25	4.033±0.974^d	2.885±0.794^c	0.9715±0.0311^a	0.9996
0.50	9.917±1.048^c	4.967±1.538^bc	0.8515±0.0702^b	0.9993
0.75	16.004±1.411^b	10.154±3.192^b	0.8374±0.0386^b	0.9975
1.00	30.405±3.827^a	37.848±7.166^a	0.5635±0.0794^c	0.9967

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表 4 IOF浓度对SOP乳液储藏稳定性的影响

Table 4. The effect of IOF concentration on the storage stability of SOP emulsion

IOF浓度（wt%）	乳液平均粒径（μm）
IOF浓度（wt%）	0 d	7 d	15 d	30 d	∆_30-0（%）
0	4.81±0.19^Ed	5.96±0.04^Ec	8.13±0.04^Eb	11.49±0.15^Ea	138.82
0.25	6.34±0.21^Dd	9.51±0.18^Dc	11.08±0.19^Db	12.64±0.11^Da	99.37
0.50	13.98±0.45^Cd	15.71±0.63^Cc	18.69±0.52^Cb	22.09±0.46^Ca	58.03
0.75	18.49±0.72^Bd	19.3±0.31^Bc	21.74±0.44^Bb	23.38±0.35^Ba	26.43
1.00	26.09±0.82^Ad	26.49±0.39^Ac	26.90±0.06^Ab	28.58±0.27^Aa	9.56
注：同列不同大写字母表示有显著性（P<0.05）；同行不同小写字母表示有显著性（P<0.05）。

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表 5 不同IOF浓度下pH和盐离子对SOP乳液的ζ-电位和平均粒径的影响

Table 5. The effect of pH and salt ions on the ζ-potential and average particle size of SOP emulsion under IOF concentration

IOF浓度（wt%）	乳液电位（mV）			乳液平均粒径（μm）
IOF浓度（wt%）	pH=7	pH=5	pH=7+ 0.1 mol/L NaCl	pH=7	pH=5	pH=7+ 0.1 mol/L NaCl
0	−35.2±0.3^eA	−23.9±0.3^eC	−30.7±0.2^aB	4.81±0.19^eE	17.62±0.24^dD	5.02±0.45^eE
0.25	−40.2±0.2^dA	−24.8±0.1^dC	−29.3±0.6^bB	6.34±0.21^dE	21.93±0.13^cD	6.62±0.53^dE
0.5	−42.0±0.2^cA	−26.0±0.5^cD	−29.0±0.4^bB	13.98±0.45^cF	27.34±0.51^bC	15.27±0.75^cE
0.75	−43.3±0.3^bA	−27.1±0.1^bD	−28.5±0.3^bC	18.49±0.72^bF	32.84±0.50^aB	19.84±0.18^bE
1	−43.9±0.2^aA	−28.3±0.4^aC	−28.9±0.4^bC	26.09±0.82^aD	33.28±0.22^aB	28.41±0.36^aC
注：同行不同大写字母表示有显著性（P<0.05）；同列不同小写字母表示有显著性（P<0.05）。

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