Abstract: The aim of this study is to establish an ion chromatography method for simultaneous determination of glucose, fructose, arabinose, galactose, mannose and ribose in beef hydrolysates. The pretreatment and chromatography conditions were optimized. Finally, the protein was precipitated by 1% trichloroacetic acid and polysaccharide was hydrolyzed by 4 mol/L sulfuric acid. The CarboPac^TM PA20 column was selected for separation. 5 mmol/L sodium hydroxide solution was used as the eluent for gradient elution, with the detection of pulse amperometric detector(PAD). The results showed that the six sugars could be separated completely under the above conditions, the linear relationship of glucose and other sugars within the range of 0.25~2.50 μg/mL and 0.025~0.25 μg/mL, respectively, was good. And the correlation coefficient was more than 0.998. The RSD of method was within 2.3%~5.5%, and the recovery was within 79.6%~116.5%. The method has the advantages of simple operation, high sensitivity and good resolution, and can be used to determine the content of sugars in beef hydrolysates accurately and rapidly.