四种牛羊乳粉脂质的比较

赖敏 吴桐 谢奎 李静 刘小如 邓泽元

赖敏,吴桐,谢奎,等. 四种牛羊乳粉脂质的比较[J]. 食品工业科技,2021,42(22):63−72. doi:  10.13386/j.issn1002-0306.2021020193
引用本文: 赖敏,吴桐,谢奎,等. 四种牛羊乳粉脂质的比较[J]. 食品工业科技,2021,42(22):63−72. doi:  10.13386/j.issn1002-0306.2021020193
LAI Min, WU Tong, XIE Kui, et al. Comparison of Lipids in Four Kinds of Milk Powder of Cow and Goat[J]. Science and Technology of Food Industry, 2021, 42(22): 63−72. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021020193
Citation: LAI Min, WU Tong, XIE Kui, et al. Comparison of Lipids in Four Kinds of Milk Powder of Cow and Goat[J]. Science and Technology of Food Industry, 2021, 42(22): 63−72. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021020193

四种牛羊乳粉脂质的比较

doi: 10.13386/j.issn1002-0306.2021020193
详细信息
    作者简介:

    赖敏(1997−)(ORCID:0000-0001-6949-4385),女,硕士研究生,研究方向:营养与食品卫生学,E-mail:2477747664@qq.com

    通讯作者:

    邓泽元(1963−)(ORCID:0000-0001-5650-1507),男,博士,教授,研究方向:食品营养与食品新资源开发,E-mail:273736939@qq.com

  • 中图分类号: TS222.1

Comparison of Lipids in Four Kinds of Milk Powder of Cow and Goat

  • 摘要: 目的:比较分析普通牛、羊乳粉和有机牛、羊乳粉的脂质特点。方法:以普通牛乳粉、普通羊乳粉、有机牛乳粉和有机羊乳粉为研究对象,采用氯仿-甲醇法提取乳粉中的脂质,氨丙基硅胶固相萃取小柱分离得甘油三酯和磷脂,利用猪胰脂酶专一水解甘油三酯分子的1,3位脂肪酸得Sn-2甘油一酯,磷脂酶A2水解磷脂得Sn-2脂肪酸,然后通过气相色谱分析甘油三酯和磷脂的脂肪酸组成和含量及其位置分布,超高液相色谱联用质谱分析甘油三酯构型,高效液相色谱测定磷脂分子组成及含量和胆固醇含量。结果:羊乳粉脂质含量、甘油三酯含量和磷脂含量均高于牛乳粉,且普通乳粉较有机乳粉高;乳粉中饱和脂肪酸主要为棕榈酸,单不饱和脂肪酸主要为油酸,且普通羊乳粉含量显著高于其余三种乳粉(P<0.05);n-6系列多不饱和脂肪酸普通羊乳粉中含量最高,n-3系列多不饱和脂肪酸在有机羊乳粉中含量最高。四种乳粉Sn-2位脂肪酸为饱和脂肪酸,且月桂酸、肉豆蔻酸和棕榈酸更倾向于分布在甘油三酯Sn-2位上,棕榈酸也更倾向于分布在磷脂Sn-2位上。四种乳粉的中链甘油三酯(Medium Chain Triglycerides,MCT)主要由Ca-Ca-Ca(Ca:C10:0)和Ca-Ca-La(La:C12:0)组成,羊乳粉较牛乳粉高;中长链甘油三酯(Medium-and Long-chain Triglycerides,MLCT)含量较高的为Ca-Ca-P(P:C16:0)、Ca-Ca-O(O:C18:0)和La-M-P(M:C14:0),羊乳粉MLCT含量显著高于牛乳粉(P<0.05);LCT含量最高的为O-P-O(O:C18:0)。结论:羊乳粉的脂质比牛乳粉更易被吸收。与普通乳粉相比,有机乳粉安全更有保障,且有机乳粉含有更高的n-3 PUFA,营养价值更高。
  • 图  1  主要脂肪酸在不同乳粉中甘油三酯中Sn-2位置分布

    Figure  1.  Distribution of sn-2 in triglycerides of different milk powders

    注:组内不同字母之间表示差异显著(P<0.05);图2~图3同。

    图  2  主要脂肪酸在不同乳粉中磷酯中Sn-2位置分布

    Figure  2.  Distribution of sn-2 in phosphate esters of different milk powders

    图  3  磷脂分子组成及相对含量

    Figure  3.  Molecular composition and relative content of phospholipids

    表  1  不同乳粉脂质组成及其含量(g/100 g乳粉)

    Table  1.   Lipid composition and content of different milk powder (g/100 g milk powder)

    脂质类别普通牛乳粉普通羊乳粉有机牛乳粉有机羊乳粉
    总脂质25.195±0.064b29.535±0.785a19.690±0.325d23.385±0.148c
    甘油三酯21.384±0.636b24.335±0.307a16.527±0.338d19.272±0.400c
    磷脂0.182±0.004b0.213±0.003a0.141±0.003c0.206±0.024ab
    胆固醇0.017±0.000b0.020±0.001a0.018±0.000ab0.018±0.001ab
    注:同行不同字母之间表示差异显著(P<0.05)。
    下载: 导出CSV

    表  2  粗脂总脂肪酸组成及绝对含量(g/100 g乳粉)

    Table  2.   Composition and absolute content of total fatty acids in crude fat (g/100 g milk powder)

    脂肪酸普通牛乳粉普通羊乳粉有机牛乳粉有机羊乳粉
    C10:00.054±0.068b0.262±0.424b0.099±0.040b0.516±0.256a
    C11:00.006±0.002b0.012±0.009b0.004±0.000b0.016±0.003a
    C12:00.630±0.161b1.188±0.328a0.552±0.011b0.909±0.043ab
    C13:00.043±0.006a0.048±0.009a0.029±0.000b0.047±0.001a
    C14:03.128±0.070b3.704±0.013a2.332±0.012d2.698±0.027c
    C15iso0.089±0.001a0.064±0.001b0.065±0.000b0.058±0.001c
    C15ai0.173±0.000a0.120±0.000b0.112±0.001c0.092±0.003d
    C15:00.370±0.004a0.353±0.005b0.247±0.000d0.290±0.005c
    C16:08.372±0.095b10.553±0.326a6.340±0.044d6.853±0.080c
    C17:00.202±0.001c0.236±0.010a0.169±0.002d0.220±0.004b
    C18:02.916±0.043bc3.013±0.115b2.877±0.012c3.213±0.027a
    ΣSFA15.982±0.196b19.552±0.317a12.826±0.033d14.913±0.168c
    C14:1 9c0.336±0.009a0.071±0.002c0.179±0.001b0.050±0.002d
    C16:1 9c0.460±0.010a0.274±0.009b0.317±0.048b0.151±0.004c
    C17:1 10c0.022±0.004ab0.027±0.002a0.016±0.001b0.018±0.005b
    C18:1 6-9c5.787±0.127c7.053±0.214a4.566±0.030d6.051±0.106b
    C18:1 11c0.108±0.005b0.169±0.008a0.106±0.005b0.099±0.002b
    ΣcisMUFA6.712±0.137b7.594±0.228a5.183±0.021d6.369±0.108c
    C16:1 9t0.176±0.007a0.148±0.006b0.117±0.001c0.115±0.002c
    C18:1 9~11t1.226±0.010a0.858±0.041b0.737±0.024c0.711±0.020c
    ΣtransMUFA1.402±0.017a1.006±0.047b0.854±0.024c0.826±0.021c
    ΣMUFA8.114±0.154b8.600±0.275a6.038±0.040d7.195±0.128c
    C18:2 9t12t0.096±0.004a0.105±0.007a0.045±0.003c0.079±0.008b
    ΣtransPUFA0.096±0.004a0.105±0.007a0.045±0.003c0.079±0.008b
    Σtrans1.498±0.013a1.111±0.048b0.900±0.027c0.905±0.025c
    C18:2 n-6 9c12c0.234±0.002d0.836±0.018a0.312±0.007c0.686±0.020b
    C20:3 n-60.015±0.001nd0.016±0.001nd
    C22:2 n-60.028±0.001a0.012±0.001b0.013±0.000b0.013±0.001b
    Σn-6PUFA0.277±0.003d0.848±0.019a0.342±0.006c0.699±0.022b
    C18:3 n-30.179±0.003c0.146±0.003d0.197±0.003b0.230±0.014a
    C20:3 n-30.020±0.002b0.042±0.002a0.020±0.001b0.039±0.002a
    C20:5 n-30.025±0.001a0.014±0.001d0.023±0.000b0.019±0.001c
    C22:5 n-30.036±0.000b0.026±0.003d0.031±0.001c0.040±0.001a
    C22:6 n-30.015±0.0060.008±0.0010.004±0.0010.015±0.001
    Σn-3PUFA0.300±0.042ab0.257±0.040b0.275±0.002b0.343±0.018a
    ΣPUFA0.672±0.039c1.210±0.055a0.663±0.005c1.121±0.039b
    ΣCLA0.426±0.009a0.172±0.004b0.163±0.004bc0.156±0.002c
    注:nd表示未检出;同行不同字母之间表示差异显著(P<0.05);表3~表4同。
    下载: 导出CSV

    表  3  甘油三酯和磷脂脂肪酸组成及绝对含量(g/100 g乳粉)

    Table  3.   Composition and absolute content of triglyceride and phospholipid fatty acids (g/100 g milk powder)

    脂肪酸甘油三酯 磷脂
    普通牛乳粉普通羊乳粉有机牛乳粉有机羊乳粉普通牛乳粉普通羊乳粉有机牛乳粉有机羊乳粉
    C10:00.067±0.016b0.272±0.133a0.107±0.068ab0.198±0.116ab ndndndnd
    C11:00.005±0.000b0.012±0.002a0.004±0.001b0.010±0.003andndndnd
    C12:00.607±0.014c1.175±0.050a0.471±0.043d0.703±0.072b0.0007±0.00000.0007±0.00030.0003±0.00000.0006±0.0003
    C13:00.040±0.001b0.045±0.001a0.024±0.001d0.037±0.002cndndndnd
    C14:02.626±0.017b3.040±0.019a1.913±0.008d2.197±0.011c0.0068±0.0010a0.0029±0.0002b0.0046±0.0006b0.0035±0.0004b
    C15iso0.075±0.001a0.052±0.001b0.052±0.001b0.047±0.001c0.0003±0.0000ndndnd
    C15ai0.142±0.001a0.096±0.001b0.090±0.002c0.075±0.001d0.0005±0.0001ndndnd
    C15:00.306±0.003a0.283±0.004b0.202±0.003d0.235±0.002c0.0014±0.0002a0.0009±0.0001b0.0010±0.0000b0.0010±0.0000b
    C16:06.982±0.029b8.453±0.108a5.243±0.078d5.650±0.065c0.0513±0.0010b0.0627±0.0002a0.0442±0.0017c0.0603±0.0019a
    C17:00.169±0.001c0.190±0.001a0.140±0.006d0.182±0.003b0.0010±0.0002bc0.0013±0.0001ab0.0008±0.0001b0.0014±0.0000a
    C18:02.408±0.015b2.364±0.048b2.347±0.068b2.642±0.048a0.0366±0.0053ab0.0331±0.0031ab0.0288±0.0003b0.0392±0.0012a
    ΣSFA13.427±0.076b15.981±0.064a10.593±0.093d11.977±0.093c0.0984±0.0033a0.1015±0.0030a0.0798±0.0026b0.1060±0.0032a
    C14:19c0.288±0.000a0.058±0.001c0.150±0.004b0.039±0.001dndndndnd
    C16:19c0.487±0.002a0.329±0.003b0.312±0.006c0.206±0.002d0.0017±0.0004a0.0007±0.0000b0.0006±0.0000b0.0007±0.0000b
    C17:110c0.020±0.004b0.025±0.002a0.015±0.000c0.021±0.001ab0.0026±0.0000a0.0016±0.0000b0.0009±0.0000c0.0019±0.0003b
    C18:16-9c4.996±0.027c5.924±0.075a3.957±0.075d5.216±0.054b0.0419±0.0028b0.0630±0.0050a0.0163±0.0039c0.0398±0.0011b
    C18:111c0.102±0.001b0.157±0.004a0.099±0.003b0.086±0.027b0.0009±0.0002b0.0018±0.0001a0.0009±0.0004b0.0011±0.0002b
    ΣcisMUFA5.893±0.031b6.494±0.072a4.533±0.085d5.568±0.056c0.0470±0.0034b0.0671±0.0050a0.0186±0.0043c0.0436±0.0010b
    C16:19t0.143±0.002a0.108±0.003b0.095±0.003c0.092±0.002c0.0009±0.00010.0009±0.00000.0008±0.00010.0008±0.0000
    C18:19~11t0.999±0.037a0.601±0.022b0.576±0.002b0.524±0.027c0.0102±0.0019b0.0095±0.0014b0.0118±0.0009ab0.0150±0.0010a
    ΣtransMUFA1.142±0.035a0.709±0.022b0.671±0.001b0.616±0.028c0.0111±0.0018b0.0104±0.0013b0.0126±0.0009ab0.0158±0.0010a
    ΣMUFA7.035±0.064b7.202±0.053a5.204±0.086d6.184±0.085c0.0581±0.0016b0.0774±0.0037a0.0312±0.0034b0.0594±0.0020c
    C18:29t12t0.078±0.004a0.076±0.001a0.039±0.003c0.059±0.002b0.0006±0.0000b0.0010±0.0000and0.0008±0.0001ab
    ΣtransPUFA0.078±0.004a0.076±0.001a0.039±0.003c0.059±0.002b0.0006±0.0000b0.0010±0.0000and0.0008±0.0001ab
    Σtrans1.220±0.037a0.785±0.021b0.710±0.003c0.675±0.027c0.0117±0.0018b0.0114±0.0013b0.0126±0.0009b0.0166±0.0011a
    C18:2n-6 9c12c0.194±0.003d0.704±0.015a0.276±0.004c0.601±0.008b0.0057±0.0014b0.0142±0.0018a0.0021±0.0004c0.0080±0.0001b
    C20:2n-6ndndndnd0.0141±0.0074b0.0131±0.0030b0.0254±0.0013a0.0269±0.0010a
    C20:3n-60.011±0.000nd0.013±0.001ndndndndnd
    C22:2n-60.020±0.000a0.008±0.001c0.010±0.000b0.010±0.000b0.0004±0.0001ab0.0005±0.0001a0.0003±0.0000b0.0004±0.0001ab
    Σn-6PUFA0.225±0.003d0.718±0.016a0.299±0.003c0.616±0.009b0.0201±0.0060b0.0278±0.0011ab0.0278±0.0008ab0.0353±0.0012a
    C18:3n-30.157±0.001c0.126±0.001d0.168±0.004b0.202±0.003a0.0016±0.0007a0.0013±0.0000ab0.0004±0.0000b0.0012±0.0001ab
    C20:3n-30.015±0.000c0.034±0.002a0.017±0.001b0.033±0.000a0.0007±0.0000bc0.0016±0.0002a0.0004±0.0000c0.0010±0.0001b
    C20:5n-30.021±0.001a0.013±0.002c0.019±0.000a0.016±0.001b0.0006±0.0001a0.0005±0.0001ab0.0003±0.0001b0.0005±0.0001ab
    C22:5 n-30.029±0.000a0.020±0.001c0.025±0.001b0.030±0.001a0.0009±0.0001b0.0014±0.0000a0.0004±0.0001c0.0014±0.0000a
    C22:6 n-30.019±0.0050.017±0.0050.014±0.0060.013±0.0010.0010±0.0003a0.0005±0.0001b0.0006±0.0001ab0.0005±0.0000b
    Σn-3PUFA0.241±0.005b0.210±0.005c0.244±0.009b0.294±0.005a0.0048±0.0011a0.0052±0.0004a0.0022±0.0001b0.0046±0.0002a
    ΣPUFA0.544±0.011d1.004±0.019a0.582±0.011c0.969±0.013b0.0255±0.0048c0.0340±0.0007ab0.0300±0.0008bc0.0406±0.0012a
    ΣCLA0.379±0.002a0.148±0.002b0.148±0.000b0.143±0.004cndndndnd
    下载: 导出CSV

    表  4  甘油三酯和磷酯Sn-2位脂肪酸组成及相对含量(%总脂肪)

    Table  4.   Composition and relative content of sn-2 fatty acids in triglycerides and phospholipids (%total fat)

    脂肪酸甘油三酯Sn-2 磷脂Sn-2
    普通牛乳粉普通羊乳粉有机牛乳粉有机羊乳粉普通牛乳粉普通羊乳粉有机牛乳粉有机羊乳粉
    C10:00.367±0.3740.514±0.5070.505±0.1090.515±0.358 ndndndnd
    C12:03.700±1.2215.559±0.7703.537±0.0144.484±0.2260.470±0.1370.515±0.1460.489±0.0630.505±0.087
    C13:00.166±0.0380.179±0.0260.155±0.0060.216±0.013ndndndnd
    C14:021.859±2.96921.605±0.03119.277±0.35320.921±0.0631.246±0.198a0.581±0.100b1.328±0.310a0.619±0.016b
    C15iso0.561±0.050a0.309±0.014c0.470±0.001b0.409±0.026bndndndnd
    C15ai1.335±0.154a0.772±0.008b0.959±0.004b0.808±0.007bndndndnd
    C15:02.061±0.198a1.451±0.000b1.669±0.005b1.625±0.014b0.388±0.0920.311±0.0000.386±0.0680.289±0.003
    C16:046.571±3.184a40.178±0.043b41.151±0.214b35.369±0.434c36.704±0.412b37.547±0.301a36.450±0.104b35.96±0.157b
    C17:01.011±0.101b0.818±0.031c0.971±0.009b1.073±0.025a0.379±0.1050.266±0.0670.926±1.0231.401±1.142
    C18:08.551±0.094b7.729±0.078c8.565±0.147b10.282±0.303a44.303±1.13846.174±0.67243.746±1.02743.121±2.359
    ΣSFA86.182±8.38379.131±1.33177.259±0.34075.701±1.27683.489±0.194ab85.394±1.087a83.325±0.298ab81.896±1.448b
    C14:19c0.328±0.3460.123±0.0010.616±0.0330.143±0.023ndndndnd
    C16:19c1.019±0.376ab0.752±0.023b1.412±0.025a0.665±0.057bndndndnd
    C17:110cndndndnd4.096±0.2584.651±0.7914.324±0.2944.575±0.088
    C18:16-11c7.919±6.366b14.747±0.990ab15.440±0.283ab17.419±0.998a10.308±0.0798.042±1.6489.782±0.07810.687±1.136
    ΣcisMUFA9.265±7.08715.621±1.01317.468±0.22518.227±1.07714.404±0.178ab12.693±0.857b14.106±0.216ab15.263±1.225a
    C16:19t0.781±0.107a0.509±0.098b0.681±0.050ab0.607±0.006abndndndnd
    C18:19~11t1.937±1.1271.933±0.0472.135±0.0812.066±0.041ndndndnd
    ΣtransMUFA2.719±1.2342.442±0.1462.816±0.1312.673±0.046ndndndnd
    ΣMUFA11.984±8.32218.063±1.15820.284±0.35620.900±1.12414.404±0.178ab12.693±0.857b14.106±0.216ab15.263±1.225a
    Σtrans2.719±1.2342.442±0.1462.816±0.1312.673±0.046ndndndnd
    C18:3n-30.250±0.181b0.301±0.008b0.575±0.038a0.579±0.002a0.475±0.033a0.230±0.034c0.492±0.011a0.321±0.004b
    Σn-3PUFA0.250±0.181b0.301±0.008b0.575±0.038a0.579±0.002a0.475±0.033a0.230±0.034c0.492±0.011a0.321±0.004b
    C18:2n-6 9c12c0.628±0.406c2.232±0.181a1.515±0.016b2.597±0.180a1.632±0.339b1.683±0.264b2.076±0.070ab2.520±0.227a
    C22:2n-61.228±0.682a0.291±0.001ab0.367±0.038ab0.223±0.026bndndndnd
    Σn-6PUFA1.856±0.276b2.523±0.181a1.882±0.022b2.820±0.155a1.632±0.339b1.683±0.264b2.076±0.07ab2.520±0.227a
    ΣPUFA2.106±0.094d2.824±0.173b2.457±0.016c3.399±0.152a2.107±0.372b1.913±0.230b2.569±0.082ab2.841±0.223a
    下载: 导出CSV

    表  5  甘油三酯组成及相对含量(%总脂肪)

    Table  5.   Composition and relative content of triglycerides (%total fat)

    序号TAG保留时间(min)质荷比(m/z)CNDBECN普通牛乳粉普通羊乳粉有机牛乳粉有机羊乳粉
    1Ca-Ca-Ca4.206572.4881300301.104±0.138b2.16±0.316a1.217±0.044b2.458±0.040a
    2Ca-Ca-La5.732600.5181320321.621±0.139b2.418±0.366a1.608±0.062b2.676±0.271a
    3ΣMCT2.724±0.278b4.578±0.682a2.825±0.079b5.134±0.282a
    4Ca-Ca-Mo6.045626.5329341320.653±0.050b0.846±0.187a0.442±0.024c1.003±0.049a
    5Ca-Ca-M7.723628.5507340343.894±0.374a3.319±0.111bc3.673±0.112ab3.119±0.082c
    6Ca-Ca-P9.618656.5821360367.297±0.549a6.99±0.070a7.52±0.163a6.073±0.263b
    7Ca-Ca-O10.255682.5977381366.805±0.136a5.949±0.124b7.352±0.370a6.236±0.171b
    8Ca-Mo-Mo8.682680.58382340.137±0.022c0.363±0.064b0.105±0.004c0.550±0.040a
    9La-La-P14.569712.6439400404.695±0.7255.312±0.035.060±0.1945.450±0.566
    10La-La-Po12.679710.6292401380.298±0.003c0.720±0.045a0.245±0.018c0.658±0.026b
    11La-M-P17.241740.6751420424.730±0.161c6.382±0.387a4.418±0.152c5.795±0.066b
    12La-M-Po15.024738.6597421401.068±0.089b1.375±0.050a0.837±0.025c1.340±0.022a
    13La-M-S19.99768.7076440441.798±0.025ab1.633±0.006b1.726±0.140b1.977±0.090a
    14Ca-P-S19.99768.7076440442.818±0.049b3.475±0.128a2.972±0.157b3.261±0.278ab
    15La-P-P19.99768.7076440442.818±0.049b3.475±0.128a2.972±0.157b3.261±0.278ab
    16La-Po-O18.094792.7071462422.461±0.195c2.957±0.063b2.166±0.126d3.807±0.004a
    17ΣMLCT39.470±1.285b42.796±0.409a39.488±0.400b42.532±0.217a
    18M-M-Po17.744766.6915441421.143±0.017a0.579±0.032c0.764±0.045b0.498±0.060c
    19M-P-Po20.236794.7234461442.087±0.010a1.597±0.090c1.796±0.027b1.216±0.053d
    20M-P-P22.372796.738460462.565±0.09a2.628±0.134a2.404±0.140a1.955±0.044b
    21M-Po-O20.975820.7402482442.432±0.015a1.842±0.115b2.165±0.082ab2.089±0.355ab
    22M-P-O22.876822.7555481463.730±0.058a3.313±0.200ab3.522±0.330a2.842±0.073b
    23M-P-S25.131824.7707480482.231±0.007a2.187±0.081a2.224±0.087a1.448±0.082b
    24P-P-O25.352850.7868501483.004±0.189a2.75±0.209a2.806±0.093a2.234±0.074b
    25Po-P-Ln19.584844.7389504420.18±0.028ab0.057±0.013c0.202±0.022a0.150±0.005b
    26M-O-L21.228846.7554503441.178±0.106a0.506±0.007d0.917±0.018b0.689±0.044c
    27M-O-O23.56848.7718502463.567±0.015a2.601±0.040c3.502±0.053a2.941±0.081b
    28Po-P-O23.56848.7718502463.567±0.015a2.601±0.040c3.502±0.053a2.941±0.081b
    29O-P-Ma26.752864.8018511490.717±0.282a0.337±0.013b0.547±0.016ab0.423±0.014b
    30S-P-Ma28.409866.8167510510.151±0.027b0.097±0.002b0.210±0.035a0.140±0.010b
    31Po-O-L22.254872.7708524440.343±0.026a0.255±0.036b0.375±0.037a0.270±0.017b
    32O-P-L24.167874.7869523461.449±0.119a1.047±0.113b1.361±0.065a1.141±0.075b
    33O-P-O25.863876.8027522483.942±0.166a3.380±0.325b4.055±0.174a3.350±0.035b
    34O-P-S27.891878.8179521501.741±0.056bc3.380±0.325a2.013±0.071b1.601±0.030c
    35O-L-L22.604898.787545440.201±0.019b0.206±0.028b0.251±0.016a0.258±0.009a
    36O-O-O26.183902.8178543482.860±0.178a2.082±0.072b2.820±0.111a2.712±0.046a
    37S-O-L26.183902.8178543482.860±0.178a2.082±0.072b2.820±0.111a2.712±0.046a
    38S-O-O28.21904.8333542501.928±0.242b1.117±0.030c2.268±0.043a2.030±0.093ab
    39S-S-O30.108906.8482541520.804±0.111b0.444±0.001c1.099±0.156a1.105±0.085a
    40ΣLCT42.679±1.563a35.088±0.272b41.623±0.370a34.746±0.401b
    注:含量为占总脂肪的百分比; Ca, C10:0; La, C12:0; M, C14:0; Mo, C14:1; P, C16:0; Po, C16:1; Ma,C17:0;S,C18:0; O, C18:1; L, C18:2;Ln, C18:3;MCT,中链甘油三酯;MLCT,中长链甘油三酯;LCT,长链甘油三酯。同行不同字母之间表示差异显著(P<0.05)。
    下载: 导出CSV
  • [1] FONTECHA J, RODRIGUEZ-ALCALA L M, CALVO M V, et al. Bioactive Milk Lipids[J]. Current Nutrition & Food Science,2011,7(3):155−159.
    [2] 曾令平, 张继东. 结构脂质及其在乳制品中的应用[J]. 中国乳品工业,2010,38(10):39−42. [ZENG Lingping, ZHANG Jidong. Introduction and application of structured lipids in dairy products[J]. China Dairy Industry,2010,38(10):39−42. doi:  10.3969/j.issn.1001-2230.2010.10.011
    [3] CONTARINI G, POVOLO M. Phospholipids in milk fat: Composition, biological and technological significance, and analytical strategies[J]. International Journal of Molecular Sciences,2013,14(2):2808−2831. doi:  10.3390/ijms14022808
    [4] 吕晓玲 王淼. 食品生物化学[M]. 北京: 中国轻工业出版社, 2009.

    LV Xiaoling, WANG Miao. Food Biochemistry [M]. Beijing: China Light Industry Press, 2009.
    [5] 王萍, 张银波, 江木兰. 多不饱和脂肪酸的研究进展[J]. 中国油脂,2008,33(12):42−46. [WANG P, ZHANG Y B, JIANG M L. Research progress of polyunsaturated fatty acids[J]. China Oils and Fats,2008,33(12):42−46. doi:  10.3321/j.issn:1003-7969.2008.12.011
    [6] BENJAMIN S, SPENER F. Conjugated linoleic acids as functional food: an insight into their health benefits[J]. Nutrition & Metabolism,2009,6(1):36−36.
    [7] 董世华. 羊奶的营养价值[J]. 食品与健康,2001(4):19. [DONG Shihua. Nutritional value of goat milk[J]. Food and Health,2001(4):19.
    [8] 李静, 邓泽元, 范亚苇, 等. 几种乳制品中脂肪酸的特点[J]. 食品工业科技,2007,28(11):221−223. [LI JING, DENG Zeyuan, FAN Yawei, et al. Characteristics of fatty acids in several dairy products[J]. Food Industry Science and Technology,2007,28(11):221−223. doi:  10.3969/j.issn.1002-0306.2007.11.074
    [9] 翁晨, 邓泽元, 李静. 乳制品脂质组成的比较[J]. 食品科学,2020,41(4):149−156. [WENG Chen, DENG Zeyuan, LI Jing. Comparison of lipid compositions of several dairy products[J]. Food Science,2020,41(4):149−156. doi:  10.7506/spkx1002-6630-20190403-046
    [10] WENG C, DENG Z Y, ZHANG N, et al. Lipid profiles of Chinese soft-shell turtle eggs (Pelodiscus sinensis)[J]. Journal of Food Composition and Analysis,2020,94:103627. doi:  10.1016/j.jfca.2020.103627
    [11] KALUZNY M A, DUNCAN L A, MERRITT M V, et al. Rapid separation of lipid classes in high yield and purity using bonded phase columns[J]. Journal of Lipid Research,1985,26(1):135−140. doi:  10.1016/S0022-2275(20)34412-6
    [12] LEI, LI J, LI G Y, et al. Stereospecific analysis of triacylglycerol and phospholipid fractions of five wild freshwater fish from poyang lake[J]. Journal of Agricultural and Food Chemistry,2012,60(7):1857−1857. doi:  10.1021/jf204584t
    [13] ICHIHARA K, SHIBAHARA A, YAMAMOTO K, et al. An improved method for rapid analysis of the fatty acids of glycerolipids[J]. Lipids,1996,31(5):535−539. doi:  10.1007/BF02522648
    [14] 胡盛本, 卓成飞, 邹古月, 等. 氢化大豆油和反刍动物油脂中甘油三酯和磷脂反式脂肪酸的位置分布[J]. 食品科学,2017,38(14):276−283. [HU Shengben, ZHUO Chengfei, ZOU Guyue, et al. Positional distribution of trans fatty acids in triglycerides and phospholipids of partially hydrogenated soybean oil and ruminant animal fat[J]. Food Science,2017,38(14):276−283. doi:  10.7506/spkx1002-6630-201714043
    [15] CRISTINA C H, DENG Z, ZHOU J, et al. Methods for analysis of conjugated linoleic acids and trans-18: 1 isomers in dairy fats by using a combination of gas chromatography, silver-ion thin-layer chromatography/gas chromatography, and silver-ion liquid chromatography[J]. Journal of AOAC International,2004,87(2):545−562. doi:  10.1093/jaoac/87.2.545
    [16] 吴琳, 刘四磊, 魏芳, 等. Florisil固相萃取法联用气相色谱测定油脂中sn-2位脂肪酸[J]. 中国油料作物学报,2015,37(2):227−233. [WU LIN, LIU SILEI, WEI FANG, et al. Determination of Sn-2 position fatty acid in oils by SPE method with Florisil extraction cartridge coupled with gas chromatography[J]. Chinese Journal of Oil Crop Sciences,2015,37(2):227−233. doi:  10.7505/j.issn.1007-9084.2015.02.016
    [17] 齐冬梅, 邹孝强, 黄健花, 等. DHA单细胞油脂的脂肪酸分布及甘油三酯组成分析[J]. 中国油脂,2018,43(9):161−167. [QI Dongmei, ZOU Xiaoqiang, HUANG Jianhua, et al. Fatty acid distribution and triglyceride composition of DHA single cell oil[J]. China Oils and Fats,2018,43(9):161−167.
    [18] MARKO, TARVAINEN, HEIKKI, et al. Regiospecific analysis of triacylglycerols by ultrahigh-performance-liquid chromatography-electrospray ionization-tandem mass spectrometry[J]. Analytical Chemistry,2019,91(21):13695−13702. doi:  10.1021/acs.analchem.9b02968
    [19] SI W, LIANG Y, MA K Y, et al. Antioxidant activity of capsaicinoid in canola oil[J]. Journal of Agricultural and Food Chemistry,2012,60(24):6230−6234. doi:  10.1021/jf301744q
    [20] Muriel M E, Antequera M T, Petron M J, et al. Stereospecific analysis of fresh and dry-cured muscle phospholipids from Iberian pigs[J]. Food Chemistry,2005,90(3):437−443. doi:  10.1016/j.foodchem.2004.05.007
    [21] ROACH J, MOSSOBA M M, YURAWECZ M P, et al. Chromatographic separation and identification of conjugated linoleic acid isomers[J]. Analytica Chimica Acta,2002,465(1):207−226.
    [22] MARCEL B, BRIGITTE F, CLAUDLA C, et al. Quantification of phospholipids in infant formula and growing up milk by high-performance liquid chromatography with evaporative light scattering detector[J]. Journal of AOAC International,2010,93(3):948−955. doi:  10.1093/jaoac/93.3.948
    [23] FIDLER N, KOLETZKO B. The fatty acid composition of human colostrum[J]. European Journal of Nutrition,2000,39(1):31−37. doi:  10.1007/s003940050073
    [24] 卓成飞, 胡盛本, 邓泽元, 等. 液态乳中胆固醇、7-脱氢胆固醇及25-羟基胆固醇的同步测定方法[J]. 中国食品学报,2019,19(4):232−240. [ZHUO Chengfei, HU SHengben, DENG Zeyuan, et al. Synchronous determination method of cholesterol, 7-dehydrocholesterol and 25-hydroxycholesterol in liquid milk[J]. Journal of Chinese Institute of Food Science and Technology,2019,19(4):232−240.
    [25] BAUER L C, SANTANA D, MACEDO M, et al. Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD[J]. Journal of the Brazilian Chemical Society,2014,25(1):161−168.
    [26] SHEILA M, INNIS. Dietary (n-3) fatty acids and brain development.[J]. The Journal of Nutrition,2007,137(4):855−859. doi:  10.1093/jn/137.4.855
    [27] 杨普煜, 张虹, 刘浚辰, 等. Sn- 2棕榈酸酯对婴幼儿健康的促进作用[J]. 食品科学技术学报,2018,36(4):41−45. [YANG PUyu, ZHANG Hong, LIU Junchen, et al. Review of Sn- 2 palmitate implications for infant health[J]. Journal of Food Science and Technology,2018,36(4):41−45. doi:  10.3969/j.issn.2095-6002.2018.04.006
    [28] ST-ONGE M P, ROSS R, PARSONS W D, et al. Medium-chain triglycerides increase energy expenditure and decrease adiposity in overweight men[J]. Obesity,2012,11(3):395−402.
    [29] 张星弛, 韩培涛, 李晓莉, 等. 中链甘油三酯的研究进展[J]. 食品研究与开发,2017,38(23):220−224. [ZHANG Xingchi, HAN Peitao, LI Xiaoli, et al. Research progress of medium chain triglycerides[J]. Food Research and Development,2017,38(23):220−224. doi:  10.3969/j.issn.1005-6521.2017.23.042
    [30] 徐文迪. 富含中长碳链甘油三酯的人乳替代脂的酶法制备[D]. 无锡: 江南大学, 2019.

    XU Wendi. Enzymatic preparation of human milk substitute fat rich in medium and long chain triglycerides [D]. Wuxi: Jiangnan University, 2019.
  • 加载中
图(3) / 表(5)
计量
  • 文章访问数:  5
  • HTML全文浏览量:  5
  • PDF下载量:  0
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-02-24
  • 网络出版日期:  2021-09-14

目录

    /

    返回文章
    返回

    重要通知

    《食品工业科技》编辑部携手万方数据开通学术不端专属检测通道,具体信息参见本刊动态。