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中国精品科技期刊2020
杨文君,刘同杰,梁曦,等. 2’-岩藻基乳糖对益生菌定殖及抗炎能力的影响[J]. 食品工业科技,2021,42(20):355−364. doi: 10.13386/j.issn1002-0306.2021020190.
引用本文: 杨文君,刘同杰,梁曦,等. 2’-岩藻基乳糖对益生菌定殖及抗炎能力的影响[J]. 食品工业科技,2021,42(20):355−364. doi: 10.13386/j.issn1002-0306.2021020190.
YANG Wenjun, LIU Tongjie, LIANG Xi, et al. Effects of 2’-Fucosyllactose on the Colonization and Anti-inflammatory Property of Probiotics[J]. Science and Technology of Food Industry, 2021, 42(20): 355−364. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020190.
Citation: YANG Wenjun, LIU Tongjie, LIANG Xi, et al. Effects of 2’-Fucosyllactose on the Colonization and Anti-inflammatory Property of Probiotics[J]. Science and Technology of Food Industry, 2021, 42(20): 355−364. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020190.

2’-岩藻基乳糖对益生菌定殖及抗炎能力的影响

Effects of 2’-Fucosyllactose on the Colonization and Anti-inflammatory Property of Probiotics

  • 摘要: 目的:探究2’-岩藻基乳糖(2’-Fucosyllactose,2’-FL)对乳酸菌和双歧杆菌增殖、粘附肠道细胞以及抗炎作用的影响。方法:以2’-FL和实验室的30株乳酸菌和双歧杆菌为研究对象,通过测定生物量、产酸和细胞粘附倍数的变化筛选出2’-FL可以增强定殖能力的菌株,再利用脂多糖诱导的RAW264.7细胞炎症模型进一步筛选出其中的潜在抗炎菌株,最后探究2’-FL和潜在抗炎菌株的联合抗炎效果。结果:30株实验菌株中,2’-FL仅能促进两歧双歧杆菌FL-276.1和FL-228.1的增殖,提高乳酸菌ML-1、FN515、FN518、FN249、ML329和双歧杆菌FL-276.1粘附Caco-2细胞的能力。其中两歧双歧杆菌FL-276.1、FL-228.1和鼠李糖乳杆菌FN518可以显著(P<0.05)降低脂多糖诱导的Raw264.7细胞炎症因子NO、TNF-α、IL-6和IL-1β的分泌。2’-FL可以显著降低NO、IL-6和IL-1β的分泌。2’-FL与上述3株菌联用具有协同抗炎作用,但协同效果具有菌株差异性,其中FL-276.1与2’-FL协同抗炎效果最好。结论:2’-FL可以提高乳酸菌和双歧杆菌的定殖能力,并协同发挥抗炎功能,但效果具有菌株差异性,这一结果可以为预防早产儿坏死性小肠结肠炎提供新的选择。

     

    Abstract: Purpose: This study aimed to investigate the effect of 2’-fucosyllactose (2’-FL) on the proliferation, adhesion to intestinal cells and anti-inflammatory ability of lactic acid bacteria and bifidobacterial. Methods: 2’-FL was used to study if the strains could utilize it for promoting proliferation or improving adhesion, by determining the biomass, acid production and variations in adhesion capacity. Then finding out the potential anti-inflammatory strains among the strains whose colonization ability was enhanced by 2’-FL, by a lipopolysaccharide (LPS)-induced macrophage RAW264.7 inflammatory model. Finally, 2’-FL and the combinations of 2’-FL and the potential anti-inflammatory strains were used to investigate their effects on the inflammation. Results: Among the thirty experimental strains, 2’-FL could promote the proliferation of Bifidobacterium bifidum (FL-276.1, FL-228.1) and improve the adhesion of lactic acid bacteria (ML-1, FN515, FN518, FN249, ML329) and bifidobacterial (FL-276.1) to Caco-2 cells. Bifidobacterium bifidum FL-276.1, Bifidobacterium bifidum FL-228.1 and Lactobacillus rhamnosus FN518 could significantly reduce the secretion of NO, TNF-α, IL-6 and IL-1β in the inflammatory model. Meanwhile, 2’-FL alone significantly reduced the secretion of NO, IL-6 and IL-1β. The combination of 2’-FL and the above three strains had a synergistic anti-inflammatory effect, which showed a strain-specific trait. Particularly, strain FL-276.1 combined with 2’-FL exerted the best anti-inflammatory effect. Conclusions: 2’-FL could promote the colonization and anti-inflammatory activity of certain lactic acid bacteria and bifidobacterial, which showed a strain-specific trait. It might provide alternative choice for the prevention of necrotizing enterocolitis (NEC) in preterm infants.

     

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