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中国精品科技期刊2020
梁竟一,胡子毅,王伟军,等. 抗幽门螺旋杆菌乳酸菌的筛选与益生特性评价[J]. 食品工业科技,2021,42(20):140−148. doi: 10.13386/j.issn1002-0306.2021020061.
引用本文: 梁竟一,胡子毅,王伟军,等. 抗幽门螺旋杆菌乳酸菌的筛选与益生特性评价[J]. 食品工业科技,2021,42(20):140−148. doi: 10.13386/j.issn1002-0306.2021020061.
LIANG Jingyi, HU Ziyi, WANG Weijun, et al. Screening of Lactic Acid Bacteria with Anti-Helicobacter pylori Activity and Evaluation of Their Probiotic Characteristics[J]. Science and Technology of Food Industry, 2021, 42(20): 140−148. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020061.
Citation: LIANG Jingyi, HU Ziyi, WANG Weijun, et al. Screening of Lactic Acid Bacteria with Anti-Helicobacter pylori Activity and Evaluation of Their Probiotic Characteristics[J]. Science and Technology of Food Industry, 2021, 42(20): 140−148. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020061.

抗幽门螺旋杆菌乳酸菌的筛选与益生特性评价

Screening of Lactic Acid Bacteria with Anti-Helicobacter pylori Activity and Evaluation of Their Probiotic Characteristics

  • 摘要: 目的:从发酵食品、乳制品和婴儿粪便中筛选具有拮抗幽门螺旋杆菌活性且益生特性良好的乳酸菌。方法:从175份样品中筛选获得39株具有良好遗传稳定性的疑似乳酸菌株,采用体外抑菌圈法和尿素酶活性试验筛选出能抑制幽门螺旋杆菌生长的9株菌,并对其中活性最强的菌株GQ1702和D1进行16S rDNA鉴定。然后,进一步评价其耐酸、耐胆盐、抗生素耐药性、抑菌性能、表面疏水性及自凝集性等益生特性。结果:筛选得到两株拮抗幽门螺旋杆菌的乳酸菌:瑞士乳杆菌GQ1702(Lactobacillus helveticus GQ1702)和植物乳杆菌D1(Lactobacillus plantarum D1),其发酵产物的抗幽门螺旋杆菌活性分别相当于浓度为0.038 mg/mL和0.034 mg/mL甲硝唑的抑制率。在pH为3的环境中培养3 h后的存活率均大于70%,在含0.3%胆盐环境中培养3 h后存活率分别为0.47%和1.08%,疏水作用力分别为25.10%和11.79%,自凝集率在24 h后分别达到75.53%和68.61%,与幽门螺旋杆菌的交互凝集率在24 h分别达到61.18%和57.25%。对常见抗生素(氯霉素、青霉素、红霉素、氨苄青霉素和头孢唑林)不耐药,对4种肠道致病菌(金黄色葡萄球菌、单核细胞增生李斯特菌、大肠杆菌O157:H7、鼠伤寒沙门氏菌)均具有抑制作用。结论:本试验筛选到具有拮抗幽门螺旋杆菌能力的植物乳杆菌D1和瑞士乳杆菌GQ1702,可为功能性乳制品的开发及研究提供菌株。

     

    Abstract: Objective: To screen lactic acid bacteria (LAB) with anti-Helicobacter pylori activity and great probiotic properties from fermented food, dairy products and infant feces. Method: A total of 39 LAB-like strains with stable curd state were screened from 175 samples. 9 Strains with anti-H. pylori activity were initially isolated by agar diffusion assay and H. pylori urease activity test and identified by 16S rDNA sequence analysis. Subsequently, the acid and bile tolerance, antibiotic resistance, antibacterial activity, cell surface hydrophobicity, and cell auto-aggregation of the selected LAB strains were determined. Results: Two probiotic strains with anti-Helicobacter pylori activity were screened: Lactobacillus helveticus GQ1702 and Lactobacillus plantarum D1, and their cell culture supernatants exhibited anti-H. pylori activity equivalent to the anti-H. pylori activity of 0.038 and 0.034 mg/mL metronidazole, respectively. The survival rates of GQ1702 and D1 were above 70% after incubation at pH3 for 3 h, the survival rates of GQ1702 and D1 after incubation at 0.3% bile salt were 0.47% and 1.08%, respectively. Cell surface hydrophobicities of GQ1702 and D1 were 25.10% and 11.79%, respectively. The self-agglutination rate reached 75.53% and 68.61% respectively after 24 hours. The auto-aggregation percentages of GQ1702 and D1 were 61.18% and 57.25%, respectively, after 24 hours. The two LAB strains were not resistant to chloramphenicol, penicillin, erythromycin, ampicillin, or cefazolin, and exhibited antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes, E. coli O157:H7, and Salmonella typhimurium. Conclusion: Lactobacillus helveticus GQ1702 and Lactobacillus plantarum D1 with anti-Helicobacter pylori activity were obtained in this experiment, which could provide a source of strains for development and research of functional dairy products.

     

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