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中国精品科技期刊2020
高志丽,王瑞雯,崔嘉纹,等. 楮果总黄酮体外抗氧化及对CCl4致脑损伤小鼠的保护作用[J]. 食品工业科技,2021,42(19):376−382. doi: 10.13386/j.issn1002-0306.2021010168.
引用本文: 高志丽,王瑞雯,崔嘉纹,等. 楮果总黄酮体外抗氧化及对CCl4致脑损伤小鼠的保护作用[J]. 食品工业科技,2021,42(19):376−382. doi: 10.13386/j.issn1002-0306.2021010168.
GAO Zhili, WANG Ruiwen, CUI Jiawen, et al. The Antioxidant Activity of Total Flavonoids from Paper Mulberry Fruits in Vitro and Its Protective Effect on CCl4-induced Brain Injury in Mice[J]. Science and Technology of Food Industry, 2021, 42(19): 376−382. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010168.
Citation: GAO Zhili, WANG Ruiwen, CUI Jiawen, et al. The Antioxidant Activity of Total Flavonoids from Paper Mulberry Fruits in Vitro and Its Protective Effect on CCl4-induced Brain Injury in Mice[J]. Science and Technology of Food Industry, 2021, 42(19): 376−382. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010168.

楮果总黄酮体外抗氧化及对CCl4致脑损伤小鼠的保护作用

The Antioxidant Activity of Total Flavonoids from Paper Mulberry Fruits in Vitro and Its Protective Effect on CCl4-induced Brain Injury in Mice

  • 摘要: 目的:探讨楮果总黄酮体外抗氧化活性及对四氯化碳(carbon tetrachloride,CCl4)致脑氧化损伤小鼠的保护作用。方法:通过体外模拟建立过氧化氢(hydrogen peroxide,H2O2)诱导红细胞溶血、肝、脑匀浆脂质过氧化、Fe2+-Vc诱导肝线粒体肿胀的生物膜实验体系和体外总抗氧化能力实验,研究楮果总黄酮的体外抗氧化活性。同时,建立CCl4诱导小鼠脑组织氧化损伤模型,将60只昆明小鼠分为空白组、模型组、阳性对照组(水飞蓟素,0.2 g/kg)和楮果总黄酮各剂量(0.15、0.3、0.6 g/kg)组,灌胃给药,隔天腹腔注射CCl4花生油溶液(体积比1:1)2 mL/kg造模,持续14 d。通过测定氧化应激参数丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)和过氧化氢酶(catalase,CAT)水平并观察小鼠脑组织海马区病理学切片,评价观察楮果总黄酮在体内的抗氧化能力。结果:体外实验中,楮果总黄酮对小鼠红细胞溶血、肝、脑组织匀浆脂质过氧化和肝线粒体肿胀的抑制作用随浓度的增加而增强,其总抗氧化能力也随浓度的增大而增强。在CCl4脑损伤模型中,楮果总黄酮可显著提高(P<0.05)小鼠脑组织SOD、GSH-PX、CAT活性和GSH水平,显著降低(P<0.05)MDA水平,且呈剂量依赖性,同时对小鼠海马神经元的病理学变化有改善作用。结论:楮果总黄酮具有较强体外抗氧化活性,并对CCl4致小鼠脑组织氧化损伤表现出较好的保护作用,具有良好的体内抗氧化能力。

     

    Abstract: Objective: To investigate the antioxidant activity of total flavonoids from paper mulberry fruits in vitro and its effect on brain damage induced by CCl4 in mice. Method: In order to study the antioxidant activity of total flavonoids from paper mulberry fruits in vitro, a biofilm experiment system with hydrogen peroxide (H2O2) inducing erythrocyte hemolysis, lipid peroxidation of liver and brain homogenate, and Fe2+-Vc inducing swelling of liver mitochondria were established. Meanwhile, a CCl4-induced oxidative damage model was established. The in vivo study was performed on 60 KM mice divided into six groups: blank group, model group, positive control group (silymarin, 0.2 g/kg) and total flavonoids groups (0.15, 0.3, 0.6 g/kg). The corresponding dose of drugs was given by intragastric administration every day, and the CCl4 peanut oil solution (1:1, V:V) was injected intraperitoneally (2 mL/kg) every other day. The blank group was given the same volume of solvent (peanut oil) for 14 days. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-PX) and catalase (CAT) were determined and the pathological sections of hippocampus were observed to evaluate the antioxidant activity of total flavonoids in vivo. Result: The inhibitory effect of total flavonoids from paper mulberry fruits on erythrocytic hemolysis, lipid peroxidation of liver and brain homogenate and swelling of liver mitochondria increased with the increase of concentration, and its total antioxidant capacity also increased with the increase of concentration in vitro. In the CCl4 brain injury model, the total flavonoids could significantly increase the SOD, GSH-PX, CAT activity and GSH levels (P<0.05) in the brain tissues of mice, and significantly reduce the MDA level (P<0.05) in a dose-dependent manner, while improving the pathological changed of hippocampal neurons. Conclusion: The total flavonoids from paper mulberry fruits have strong antioxidant activity in vitro, and show a good protective effect on CCl4 induced oxidative injury in mice brain tissue, have good antioxidant capacity in vivo.

     

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