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中国精品科技期刊2020
韩玉泽,王兴瑞,李应霞,等. SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分[J]. 食品工业科技,2021,42(20):255−260. doi: 10.13386/j.issn1002-0306.2021010089.
引用本文: 韩玉泽,王兴瑞,李应霞,等. SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分[J]. 食品工业科技,2021,42(20):255−260. doi: 10.13386/j.issn1002-0306.2021010089.
HAN Yuze, WANG Xingrui, LI Yingxia, et al. Analysis and Identification of Volatile Profiles in Qinghai Flaxseed Oil by SPME-GC-MS[J]. Science and Technology of Food Industry, 2021, 42(20): 255−260. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010089.
Citation: HAN Yuze, WANG Xingrui, LI Yingxia, et al. Analysis and Identification of Volatile Profiles in Qinghai Flaxseed Oil by SPME-GC-MS[J]. Science and Technology of Food Industry, 2021, 42(20): 255−260. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010089.

SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分

Analysis and Identification of Volatile Profiles in Qinghai Flaxseed Oil by SPME-GC-MS

  • 摘要: 以青海不同产地的40种亚麻籽为原料,索氏抽提提取亚麻籽油,采用顶空固相微萃取结合气相色谱-质谱联用对亚麻籽油挥发性组分进行分析,通过优化搅拌速率、萃取温度、平衡时间、萃取时间、解吸时间、升温程序确定最佳固相微萃取条件及GC-MS条件。结果表明,采用DVB/CAR/PDMS萃取头,亚麻籽油SPME最佳条件为磁力搅拌速率400 r/min,萃取温度80 ℃,平衡时间20 min,萃取时间40 min,解吸时间5 min,在该萃取条件下可以检测出青海40种亚麻籽油挥发性成分共58种,醛类12种,酸类8种,醇类9种,酮类2种,酯类5种,烷烯类13种,杂环类3种,其他类6种,分离鉴定效果较好;根据聚类分析,醛类物质是将亚麻籽油样品进行区分的主要挥发性组分,该结果为亚麻籽油品质鉴定及开发利用提供了理论依据。

     

    Abstract: Flaxseed oil was prepared from 40 kinds of flaxseed of different region in Qinghai by soxhlet extraction. And the volatile profiles were analyzed by headspace sampling combined with GC-MS method. In order to obtain the best solid phase microextraction conditions and GC-MS condition, the stirring rate, extraction temperature, equilibrium time, extraction time, desorption time and heating program were optimized. The results indicated that when using DVB/CAR/PDMS fiber, the optimum extraction conditions of the volatile compounds were: 20 min equilibrium time, 40 min adsorption time at 80 ℃ , stirring intensity of 400 r/min, and 5 min desorption time. Under this extraction condition, a total of 58 volatile components of 40 kinds of flaxseeds oil in Qinghai could be detected, including 12 aldehydes, 8 acids, 9 alcohols, 2 ketones, 5 esters, 13 hydrocarbon compounds, 3 heterocycles and 6 other types, which achieved a good chromatographic separation. According to cluster analysis, aldehydes were the main volatile components that distinguished flaxseed oil samples, which would provide a theoretical basis for the quality identification and development of flaxseed oil.

     

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