SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分

韩玉泽 王兴瑞 李应霞 王淑珍 陈昀昀 王进英

韩玉泽,王兴瑞,李应霞,等. SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分[J]. 食品工业科技,2021,42(20):255−260. doi:  10.13386/j.issn1002-0306.2021010089
引用本文: 韩玉泽,王兴瑞,李应霞,等. SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分[J]. 食品工业科技,2021,42(20):255−260. doi:  10.13386/j.issn1002-0306.2021010089
HAN Yuze, WANG Xingrui, LI Yingxia, et al. Analysis and Identification of Volatile Profiles in Qinghai Flaxseed Oil by SPME-GC-MS[J]. Science and Technology of Food Industry, 2021, 42(20): 255−260. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021010089
Citation: HAN Yuze, WANG Xingrui, LI Yingxia, et al. Analysis and Identification of Volatile Profiles in Qinghai Flaxseed Oil by SPME-GC-MS[J]. Science and Technology of Food Industry, 2021, 42(20): 255−260. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021010089

SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分

doi: 10.13386/j.issn1002-0306.2021010089
基金项目: 青海省青年自然科学基金(2018-ZJ-970Q);省部共建三江源生态与高原农牧业国家重点实验室自主课题(2019-ZZ-06)
详细信息
    作者简介:

    韩玉泽(1997−),女,硕士研究生,研究方向:油脂加工与利用,E-mail:18453321393@163.com

    通讯作者:

    王进英(1989−),女,副教授,研究方向:油脂加工与利用,E-mail:wangjinying0128@126.com

  • 中图分类号: TS225.1

Analysis and Identification of Volatile Profiles in Qinghai Flaxseed Oil by SPME-GC-MS

  • 摘要: 以青海不同产地的40种亚麻籽为原料,索氏抽提提取亚麻籽油,采用顶空固相微萃取结合气相色谱-质谱联用对亚麻籽油挥发性组分进行分析,通过优化搅拌速率、萃取温度、平衡时间、萃取时间、解吸时间、升温程序确定最佳固相微萃取条件及GC-MS条件。结果表明,采用DVB/CAR/PDMS萃取头,亚麻籽油SPME最佳条件为磁力搅拌速率400 r/min,萃取温度80 ℃,平衡时间20 min,萃取时间40 min,解吸时间5 min,在该萃取条件下可以检测出青海40种亚麻籽油挥发性成分共58种,醛类12种,酸类8种,醇类9种,酮类2种,酯类5种,烷烯类13种,杂环类3种,其他类6种,分离鉴定效果较好;根据聚类分析,醛类物质是将亚麻籽油样品进行区分的主要挥发性组分,该结果为亚麻籽油品质鉴定及开发利用提供了理论依据。
  • 图  1  搅拌速率对萃取效果的影响

    Figure  1.  Effect of stirring rate on the extraction effect

    图  2  萃取温度对萃取效果的影响

    Figure  2.  Effect of extraction temperature on extraction effect

    图  3  平衡时间对萃取效果的影响

    Figure  3.  Effect of equilibration time on the extraction effect

    图  4  吸附时间对萃取效果的影响

    Figure  4.  Effect of adsorption time on extraction effect

    图  5  解吸时间对萃取效果的影响

    Figure  5.  Effect of desorption time on extraction effect

    图  6  不同升温程序下亚麻籽油挥发性成分的总离子流图

    Figure  6.  Total ion chromatograms of volatile components of linseed oil under different heating programs

    图  7  40个亚麻籽油样品聚类谱系图

    Figure  7.  Cluster pedigree map of 40 flaxseed oil samples

    表  1  亚麻籽油信息表

    Table  1.   Information of flaxseed oil

    编号来源编号来源编号来源编号来源
    S1西宁大通S11西宁湟中9S21海东互助3S31海东民和5
    S2西宁湟源S12西宁湟中10S22海东互助4S32海东民和6
    S3西宁湟中1S13西宁湟中11S23海东互助5S33海东平安1
    S4西宁湟中2S14西宁湟中12S24海东互助6S34海东平安2
    S5西宁湟中3S15西宁湟中13S25海东乐都S35海东平安3
    S6西宁湟中4S16海南贵德1S26海东民和低杆S36海东平安4
    S7西宁湟中5S17海南贵德2S27海东民和1S37海东平安5
    S8西宁湟中6S18海北门源S28海东民和2S38海东平安6
    S9西宁湟中7S19海东互助1S29海东民和3S39海东循化1
    S10西宁湟中8S20海东互助2S30海东民和4S40海东循化2
    下载: 导出CSV

    表  2  不同类别亚麻籽油挥发性成分的相对含量(%)

    Table  2.   Relative content of volatile components in different classifications of flaxseed oil (%)

    种类醛类酸类醇类酮类酯类烷烯类杂环类其他
    S19.9316.8010.885.8427.453.3525.76
    S210.6516.151.788.393.2845.881.8812.01
    S313.8222.198.861.4828.397.1718.11
    S410.355.8911.8232.988.0620.32
    S57.9110.585.326.0934.526.1229.47
    S69.569.415.711.3541.124.3328.53
    S76.995.046.2053.752.2925.74
    S812.533.338.0140.847.3627.94
    S940.1113.243.3815.443.139.947.537.25
    S1018.9513.907.079.7918.6010.5421.16
    S1113.7416.311.917.244.6333.652.1820.36
    S1223.3412.992.109.5531.255.1015.68
    S1353.6946.31
    S144.9423.258.0528.7134.96
    S1554.826.385.767.2511.4814.32
    S1618.8123.218.161.523.3027.553.6313.84
    S1728.0613.982.1115.802.0924.412.2611.32
    S1810.141.455.4823.503.5055.94
    S1911.4615.4712.131.5636.734.3218.35
    S2014.2211.503.184.6441.694.9219.87
    S2115.8616.5210.691.3934.169.5811.81
    S2240.125.6615.246.139.565.0018.30
    S2329.7626.036.396.428.883.7018.83
    S2411.059.173.8811.551.9237.963.6620.82
    S2523.9510.221.428.0530.452.0523.87
    S2611.0919.251.589.591.1824.875.7026.77
    S279.0017.083.705.772.1940.554.1317.60
    S2815.3222.226.753.5831.713.7916.64
    S298.9316.038.1719.8619.033.3724.62
    S3019.1213.4922.0217.6427.74
    S3111.3718.667.5824.634.7133.07
    S326.4512.9947.5633.00
    S336.686.2147.346.8332.95
    S348.495.088.831.1846.603.8126.02
    S3511.9812.3111.361.6837.155.3520.19
    S363.783.747.434.9044.347.8327.99
    S3713.7115.9910.0140.724.1415.44
    S3814.776.1115.9739.558.0815.83
    S399.3920.0511.8232.988.0617.72
    S4015.7018.021.237.2532.307.0418.47
    下载: 导出CSV
  • [1] Bushra S, Pang Z H, Liu X Q, et al. Structural and functional properties of raw and defatted flaxseed flour and degradation of cynogenic contents using different processing methods[J]. Journal of Food Process Engineering,2020,43(6):e13406.
    [2] Yang R N, Zang L X, Li P W, et al. A review of chemical composition and nutritional properties of minor vegetable oils in China[J]. Trends in Food Science & Technology,2018,74:26−32.
    [3] 邹仙果. 亚麻籽环肽组成、氧化结构变化及其体外抗肿瘤抗炎活性研究[D]. 南昌: 南昌大学, 2019.

    Zou X G. The profiles, structural changes during oxidation and in vitro antitumor and anti-inflammatory activities of linusorbs in flaxseed[D]. Nanchang: Nanchang University, 2019.
    [4] Han Y, Deng X, Zhang Y, et al. Antidepressant-like effect of flaxseed in rats exposed to chronic unpredictable stress[J]. Brain and Behavior,2020,10(6):e01626.
    [5] 安骏, 李昌模, 金青哲, 等. 含油溶性营养成分食用油的安全性研究[J]. 粮食与食品工业,2016,23(6):3−7, 12. [An J, Li C M, Jin Q Z, et al. Study on the safety of edible oil with oil soluble nutrients[J]. Cereal & Food Industry,2016,23(6):3−7, 12. doi:  10.3969/j.issn.1672-5026.2016.06.002
    [6] 马良, 王若兰. 顶空固相微萃取-气质联用技术在粮油食品中的应用进展[J]. 粮食与油脂,2015,28(1):6−10. [Ma L, Wang R L. Research advances on the application of HS-SPME/GC-MS technology in cereals, oils and foods[J]. Cereals & Oils,2015,28(1):6−10. doi:  10.3969/j.issn.1008-9578.2015.01.002
    [7] Zquezarau J, Verdu A, Navar R, et al. Changes in volatile compounds and sensory quality during toasting of Spanish almonds[J]. Int J Food Sci Tech,2009,44(11):2225−2233. doi:  10.1111/j.1365-2621.2009.02063.x
    [8] Lasekan O, Abbas K. Analysis of volatile flavour compounds and acrylamide in roasted Malaysian tropical almond(Terminalia catappa) nuts using supercritical fluid extraction[J]. Food Chem Toxicol,2010,48(8/9):2212−2216.
    [9] Curtis M, Kalua, Danny R, et al. Development of a headspace solid phase microextraction-gas chromatography method for monitoring volatile compounds in extended time-course experiments of olive oil[J]. Analytica Chimica Acta,2005,556(2):407−414.
    [10] Min H P, Min K J, Ju D Y, et al. Application of solid phase-microextraction(SPME) and electronic nose techniques to differentiate volatiles of sesame oils prepared with diverse roasting conditions[J]. Journal of Food Science,2011,76(1):80−88. doi:  10.1111/j.1750-3841.2010.01954.x
    [11] 周波, 孙亚娟, 王进英, 等. 茶油挥发性成分指纹图谱的构建及掺伪定量检测方法研究[J]. 中南林业科技大学学报,2017,37(9):118−123. [Zhou B, Sun Y J, Wang J Y, et al. Detection method research on adulteration of Camellia seed oil based on fingerprint of volatile components[J]. Journal of Central South University of Forestry & Technology,2017,37(9):118−123.
    [12] 秦早, 杨冉, 高桂园, 等. 顶空固相微萃取结合气质联用分析芝麻油和芝麻香精的挥发性成分[J]. 食品科学,2012,33(24):263−268. [Qin Z, Yang R, Gao G Y, et al. Analysis of volatile components of sesame oil and sesame flavor by headspace solid phase microextraction combined with GC-MS[J]. Food Science,2012,33(24):263−268. doi:  10.7506/spkx1002-6630-201224056
    [13] Dierkes G, Bongartz A, Guth H, et al. Quality evaluation of olive oil by statistical analysis of multicomponent stable isotope dilution assay data of aroma active compounds[J]. Journal of Agricultural & Food Chemistry,2012,60(1):394−401.
    [14] 于文龙, 郝楠, 吴凯晋, 等. HS-SPME-GC-MS-O联用分析不同加工工艺亚麻籽油特征香气成分[J]. 食品科学,2019,40(18):266−272. [Yu W L, Hao N, Wu K J, et al. Analysis of characteristic aroma components of flaxseed oils obtained by different processing techniques by HS-SPME-GC-MS-O[J]. Food Science,2019,40(18):266−272. doi:  10.7506/spkx1002-6630-20181009-073
    [15] 杨柳, 吴翠蓉, 朱杰丽, 等. 顶空-气质联用法鉴别油茶籽油真伪[J]. 中国粮油学报,2012,27(11):105−109, 113. [Yang L, Wu C R, Zhu J L, et al. Identification of Camellia seed oil by headspace-GC-MS[J]. Journal of The Chinese Cereals and Oils Association,2012,27(11):105−109, 113. doi:  10.3969/j.issn.1003-0174.2012.11.024
    [16] 陈超, 黄景霞, 李梦, 等. 新疆胡麻油香气萃取条件研究[J]. 食品工业科技,2014,35(2):242−246. [Chen C, Huang J X, Li M, et al. Study on extraction conditions of Xinjiang flaxseed oil aroma[J]. Science and Technology of Food Industry,2014,35(2):242−246.
    [17] 邓龙, 刘雄飞, 刘贤标, 等. GC-MS-O结合电子鼻对橄榄油挥发性成分的分析与鉴别[J]. 中国食品学报,2019,19(5):276−286. [Deng L, Liu X F, Liu X B, et al. Analysis and identification of volatile components in olive oil by GC-MS-O combined with electronic nose[J]. Journal of Chinese Institute of Food Science and Technology,2019,19(5):276−286.
    [18] 李晓颍, 王海静, 徐宁伟, 等. 顶空固相微萃取-气相色谱-质谱联用法分析欧李果实挥发性成分[J]. 中国农业科学,2019,52(19):3448−3459. [Li X Y, Wang H J, Xu N W, et al. Analysis of volatile components of plum fruit by headspace solid phase microextraction-gas chromatography-mass spectrometry[J]. Journal of Integrative Agriculture,2019,52(19):3448−3459. doi:  10.3864/j.issn.0578-1752.2019.19.014
    [19] 王丹, 王永瑞, 魏哲文, 等. 顶空固相微萃取结合GC-MS对大豆油香气成分分析[J]. 农产品加工,2020,1:53−55. [Wang D, Wang Y R, Wei Z W, et al. Analysis of soybean oil aroma components by headspace solid phase microextraction combined with GC-MS[J]. Roducts Rocessing,2020,1:53−55.
    [20] 芦鑫, 张丽霞, 王奕芳, 等. 不同品种花生乳挥发性组分分析[J]. 中国油脂,2018,43(5):139−144. [Lu X, Zhang L X, Wang Y F, et al. Analysis of volatile omponents in different varieties of peanut milk[J]. China Oils and Fats,2018,43(5):139−144. doi:  10.3969/j.issn.1003-7969.2018.05.032
    [21] 王笑园, 宋章弈, 张延琦, 等. 精炼过程对亚麻籽油风味物质的影响[J]. 食品工业科技,2016,37(18):55−59. [Wang X Y, Song Z Y, Zhang Y Q, et al. Influence of refining process on flaxseed oil flavor[J]. Science and Technology of Food Industry,2016,37(18):55−59.
    [22] Franc A, Maurizio S, Roberto S, et al. Volatile compounds in virgin olive oil: Occurrence and their relationship with the quality[J]. Journal of Chromatography A,2004,1054(1):18−27.
    [23] Wei C Q, Liu W Y, Xi W P, et al. Comparison of volatile compounds of hot-pressed, cold-pressed and solvent-extracted flaxseed oils analyzed by SPME-GC/MS combined with electronic nose: Major volatiles can be used as markers to distinguish differently processed oils[J]. J Lipid Sci Technol,2015,117:320−330. doi:  10.1002/ejlt.201400244
    [24] Li X, Gao Y, Chen Z Y, et al. Development of a chromatographic fingerprint of tobacco flavor by use of GC and GC-MS[J]. Chromatographia,2009,69(7−8):735−742. doi:  10.1365/s10337-009-0968-4
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  • 收稿日期:  2021-01-15
  • 网络出版日期:  2021-09-03
  • 刊出日期:  2021-10-11

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