不同酪蛋白磷酸肽产品理化性质与持钙能力的相关性分析

姜春秀 全威 陈洁 贺来健

姜春秀,全威,陈洁,等. 不同酪蛋白磷酸肽产品理化性质与持钙能力的相关性分析[J]. 食品工业科技,2021,42(18):292−299. doi:  10.13386/j.issn1002-0306.2020120183
引用本文: 姜春秀,全威,陈洁,等. 不同酪蛋白磷酸肽产品理化性质与持钙能力的相关性分析[J]. 食品工业科技,2021,42(18):292−299. doi:  10.13386/j.issn1002-0306.2020120183
JIANG Ginger, QUAN Wei, CHEN Jie, et al. Correlation Analysis between Properties and Calcium-holding Capacity of Different Casein Phosphopeptide Products[J]. Science and Technology of Food Industry, 2021, 42(18): 292−299. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020120183
Citation: JIANG Ginger, QUAN Wei, CHEN Jie, et al. Correlation Analysis between Properties and Calcium-holding Capacity of Different Casein Phosphopeptide Products[J]. Science and Technology of Food Industry, 2021, 42(18): 292−299. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020120183

不同酪蛋白磷酸肽产品理化性质与持钙能力的相关性分析

doi: 10.13386/j.issn1002-0306.2020120183
详细信息
    作者简介:

    姜春秀(1980−),女,本科,中级工程师,研究方向:婴幼儿辅助食品,E-mail:ginger@pigeon.cn

    通讯作者:

    贺来健(1963−),男,博士,研究方向:婴幼儿辅助食品以及婴幼儿护肤品,E-mail:ken.kaku@pigeon.cn

  • 中图分类号: TS207.3

Correlation Analysis between Properties and Calcium-holding Capacity of Different Casein Phosphopeptide Products

  • 摘要: 为探究酪蛋白磷酸肽(CPP)产品性质对其持钙能力的影响,以市面上常见的7种酪蛋白磷酸肽产品为研究对象,测定了酪蛋白磷酸肽含量、氮磷含量及氮磷比(N/P)、分子量分布、氨基酸组成,并对其理化性质与持钙能力进行相关性分析。结果表明,7种产品的酪蛋白磷酸肽含量及N/P结果分别在1.30%~18.50%和12.70~46.80之间,氨基酸含量为70.50~901.00 mg/g,且主要以脯氨酸为主,分子量分布则集中在5000 Da以下(除7号样品)。以不同添加量(0.05、0.1、0.2 g/L)将7种酪蛋白磷酸肽产品加入到模拟反应体系中测定其持钙能力,发现1、2和6号产品的持钙能力较强,7号样品的持钙能力最差。将上述结果进行主成分分析(R1=0.674,R2=0.167),结果表明酪蛋白磷酸肽产品的分子量分布、氨基酸含量以及酪蛋白磷酸肽的含量与其持钙能力强弱存在一定的关联。进一步进行相关性分析发现,分子量>10 kDa的组分含量与酪蛋白磷酸肽样品的持钙能力强弱呈现极显著的负相关性(相关系数−0.985~−0.975,P<0.01),而分子量分布类似且异亮氨酸(相关系数0.766~0.768,P<0.05)和苯丙氨酸(相关系数0.758~0.759,P<0.05)含量较高的CPP样品中则表现更强的持钙能力。本研究为评价和提高酪蛋白磷酸肽产品质量提供了一定的理论依据。
  • 图  1  7种CPP样品(添加量为0.05 g/L)阻止磷酸钙沉淀形成效果

    Figure  1.  Effect of 7 CPP samples(at 0.05 g/kg)on preventing calcium phosphate precipitation formation

    图  2  7种CPP样品(添加量为0.1 g/L)阻止磷酸钙沉淀形成效果

    Figure  2.  Effect of 7 CPP samples(at 0.1 g/kg)on preventing calcium phosphate precipitation formation

    图  3  7种CPP样品(添加量为0.2 g/L)阻止磷酸钙沉淀形成效果

    Figure  3.  Effect of 7 CPP samples(at 0.2 g/kg)on preventing calcium phosphate precipitation formation

    图  5  7种CPP样品的主成分分析得分图

    Figure  5.  Principal component analysis score plots for the 7 CPP samples

    图  6  7种CPP样品的主成分分析载荷图

    Figure  6.  Principal component analysis loadings for 7 CPP samples

    注:CHC1:CPP添加量为0.05 g/L的持钙能力测试中氢氧化钠消耗量;CHC2:CPP添加量为0.1 g/L的持钙能力测试中氢氧化钠消耗量;CHC3:CPP添加量为0.2 g/L的持钙能力测试中氢氧化钠消耗量;Mw1:分子量大于10000 Da的组分含量;Mw2:分子量5000~10000 Da的组分含量;Mw3:分子量1000~5000 Da的组分含量;Mw4:分子量小于1000 Da的组分含量;Content:CPP含量。

    表  1  乙醇钡沉淀法测定的样品中CPP含量

    Table  1.   CPP content in different samples determined by barium ethanol precipitation method

    物料编号CPP含量(%)
    111.40±1.92c
    27.68±0.08d
    314.60±1.06b
    418.50±1.39a
    58.70±1.05d
    615.20±1.06b
    71.30±0.25e
    注:同列不同小写字母表示差异显著,P<0.05;表2表4同。
    下载: 导出CSV

    表  2  CPP中N、P含量及比值

    Table  2.   N and P content and ratio in CPP

    物料编号N含量(%)P含量(%)N/P
    111.70±0.32b1.57±0.15a16.50
    212.90±0.21a0.74±0.03c38.80
    311.90±0.33b0.96±0.11b27.40
    411.80±0.47b0.81±0.04bc32.20
    512.20±0.18b0.74±0.13b36.50
    613.30±0.28a0.72±0.19b46.80
    79.01±0.46c0.71±0.22b12.70
    下载: 导出CSV

    表  3  CPP中氨基酸组成及含量(mg/g)

    Table  3.   Composition and content of amino acid in CPP(mg/g)

    氨基酸1234567
    Asp4.35±0.03d8.62±0.12a4.48±0.21cd4.84±0.11bc5.27±0.33b4.83±0.09bc5.24±0.14b
    Glu77.80±2.13b136.00±5.87a60.20±5.03c57.5±3.73c70.70±2.18b60.50±2.44c15.40±1.51d
    Ser9.35±1.96b14.50±1.07a6.93±0.83b7.25±1.01b8.69±0.53b7.63±0.72b3.98±0.50c
    Gly9.01±2.69a8.30±1.53a3.84±0.74b4.21±1.82b5.26±1.43b4.49±0.93b1.40±0.34c
    His9.21±0.85c31.50±4.75a15.50±3.99b15.0±4.28b17.20±3.86b18.00±4.04b1.61±0.41d
    Arg10.50±0.78b14.40±1.21a6.65±0.60c7.25±0.73c9.26±0.65b7.84±0.33c2.41±0.53d
    Thr28.60±3.31c71.30±8.50a31.60±4.69b33.6±4.28bc37.60±5.75b38.50±2.07b2.95±0.42d
    Ala8.61±2.15b14.00±3.77a6.70±1.09c7.03±1.00c8.43±2.23b7.67±0.80c2.21±0.13d
    Pro168.00±9.98c369.00±21.60a286.00±17.50b257.00±20.00b280.00±16.20b302.00±35.90b6.76±1.96d
    Tyr17.30±4.03b30.20±6.22a12.00±2.77b13.7±1.09b17.40±2.43b15.00±4.11b3.86±1.52c
    Val19.70±5.51b36.30±8.24a17.70±3.90b17.60±4.14b20.60±2.99b19.40±2.73b4.22±1.86c
    Met7.72±0.99b14.40±5.87a7.23±3.71b7.03±2.02b8.15±1.76b7.86±2.75b1.88±0.63c
    Cys1.77±0.01c5.30±1.77a2.95±0.40b2.83±0.58b2.25±0.66bc2.76±0.37b0.19±0.00d
    Ile20.70±5.57b41.10±7.14a20.80±3.86b20.30±6.0723.00±3.99b22.60±5.50b3.31±1.00c
    Leu15.20±1.71c63.10±8.22a31.80±6.93b30.80±7.04b34.60±3.07b34.30±4.73b6.48±0.72d
    Phe20.00±3.86b42.10±5.19a22.00±4.29b20.60±3.33b22.90±1.88b23.40±2.00b3.40±0.75c
    Lys9.10±2.23a0.85±0.14c0.62±0.08c0.58±0.13c9.92±1.44a0.58±0.00c5.13±1.11b
    总量438.00±41.00c901.00±92.20a537.00±71.40b507.00±49.50bc581.00±62.30b578.00±66.90b70.50±21.80d
    注:同行不同小写字母表示差异显著,P<0.05。
    下载: 导出CSV

    表  4  CPP中分子量分布(%)

    Table  4.   Molecular weight distribution in CPP(%)

    物料编号>10000 Da5000~10000 Da1000~5000 Da<1000 Da
    12.70±0.02d6.82±0.39d55.40±0.14a35.10±0.52b
    210.80±0.23b16.20±0.22a42.40±0.28b30.70±0.30c
    30.15±0.00f0.37±0.01f12.10±0.21c87.40±0.21a
    41.60±0.02e0.85±0.01e14.80±0.32c82.70±0.33a
    56.89±0.15c12.60±0.27b44.50±0.03b36.00±0.45b
    67.12±0.00c12.30±0.00b43.80±0.40b36.70±0.40b
    766.80±0.17a10.20±0.11c9.60±0.25d13.40±0.31d
    下载: 导出CSV

    表  5  CPP样品特性与持钙能力的相关性分析

    Table  5.   Correlation analysis of CPP sample characteristics and calcium-holding capacity

    指标丙氨酸脯氨酸酪氨酸缬氨酸甲硫氨酸异亮氨酸苯丙氨酸赖氨酸分子量
    (>10 kDa)
    分子量
    (0.5~10 kDa)
    分子量
    (0.1~0.5 kDa)
    分子量
    (<0.1 kDa)
    CPP含量
    持钙能力(添加量0.05 g/L)0.7200.6860.6690.7230.7190.7460.738−0.136−0.985**0.0100.5170.4940.746
    持钙能力(添加量0.1 g/L)0.7470.7040.70.7480.7430.768*0.759*−0.14−0.975**0.0250.5430.4610.733
    持钙能力(添加量0.2 g/L)0.7430.7030.6940.7440.740.766*0.758*−0.159−0.975**0.0170.5380.4670.743
    注:*表示显著,P<0.05;**表示极显著,P<0.01。
    下载: 导出CSV
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  • 收稿日期:  2020-12-23
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