Abstract: To explore the effect of different ratio of light on physiological reaction and main amino acid accumulation of annual Fuding-dabaicha leaves. The L1 (red light accounted for 71.8%), L2 (blue light accounted for 62.7%), L3 (red 43.1%, blue 29%, green accounted for 27.9%) and L0 (green light accounted for 82.1%) were set in artificial climate boxes with 100 μmol·m⁻²·s⁻¹ photosynthetic photon flux density and 12 h photo period. The leaf growth index were determined by nitrogen balance index instrument and the theanin, glutamate and aspartate contents were determined by an automatic amino acid analyzer. The results showed that with the increase of treatment time, the chlorophyll index and nitrogen balance index of tea leaves were significantly increased by all light treatments. Compared with L0, L1 significantly increased the nitrogen balance index of tea leaves (P<0.05), followed by L3, index of flavonoids and polyphenols content between different light treatment had no significant difference. Compared with L0, the content of theanine, glutamate and aspartate under L3 and L2 treatments increased significantly. In conclusion, L3 (an equilibrium ratio of red, blue and green light) can be considered as a suitable artificalight source for tea leaves growth and to ensure the maximun retention of main amino acid.