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中国精品科技期刊2020
杨子帆,张科,刘莹,等. 鲟鱼和草鱼鱼鳔酶溶性胶原蛋白的理化性质比较[J]. 食品工业科技,2021,42(15):27−32. doi: 10.13386/j.issn1002-0306.2020110108.
引用本文: 杨子帆,张科,刘莹,等. 鲟鱼和草鱼鱼鳔酶溶性胶原蛋白的理化性质比较[J]. 食品工业科技,2021,42(15):27−32. doi: 10.13386/j.issn1002-0306.2020110108.
YANG Zifan, ZHANG Ke, LIU Ying, et al. Comparison of Physicochemical Properties of Pepsin-Soluble Collagens from Swim Bladders of Sturgeon(Acipenser schrenckii) and Grass Carp(Ctenopharyngodon idella) [J]. Science and Technology of Food Industry, 2021, 42(15): 27−32. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110108.
Citation: YANG Zifan, ZHANG Ke, LIU Ying, et al. Comparison of Physicochemical Properties of Pepsin-Soluble Collagens from Swim Bladders of Sturgeon(Acipenser schrenckii) and Grass Carp(Ctenopharyngodon idella) [J]. Science and Technology of Food Industry, 2021, 42(15): 27−32. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110108.

鲟鱼和草鱼鱼鳔酶溶性胶原蛋白的理化性质比较

Comparison of Physicochemical Properties of Pepsin-Soluble Collagens from Swim Bladders of Sturgeon(Acipenser schrenckii) and Grass Carp(Ctenopharyngodon idella)

  • 摘要: 以鲟鱼鱼鳔和草鱼鱼鳔为原料,比较分析两种鱼鳔酶溶性胶原蛋白(PSC)的理化性质。通过胶原蛋白得率、氨基酸组成、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、紫外光谱、傅里叶红外光谱、圆二光谱、微量热仪分析两种鱼鳔PSC的组成及理化性质的差异。结果表明,鲟鱼鱼鳔基本营养成分中的粗脂肪和羟脯氨酸含量均显著高于草鱼鱼鳔(P<0.05);鲟鱼鱼鳔PSC得率80.63%显著高于草鱼鱼鳔PSC得率49.85%(P<0.05);两种鱼鳔PSC都属于I型胶原蛋白;鲟鱼鱼鳔PSC的α链相对分子质量和二聚体β链的含量低于草鱼鱼鳔PSC;氨基酸组成中鲟鱼鱼鳔PSC中的丝氨酸、蛋氨酸、异亮氨酸、亮氨酸、组氨酸和精氨酸的含量显著高于草鱼鱼鳔PSC(P<0.05),草鱼鱼鳔PSC中的丙氨酸、苯丙氨酸、赖氨酸、脯氨酸和羟脯氨酸的含量显著高于鲟鱼鱼鳔PSC(P<0.05);两种鱼鳔PSC具有相似的二级结构;鲟鱼鱼鳔PSC的变性温度29.26 ℃显著低于草鱼鱼鳔PSC变性温度36.01 ℃(P<0.05)。在两种鱼鳔PSC之间,草鱼鱼鳔PSC比鲟鱼鱼鳔PSC有更好的热稳定性,但是鲟鱼鱼鳔PSC比草鱼鱼鳔PSC有更高的得率。

     

    Abstract: Using sturgeon and grass carp swim bladder as raw materials, compared and analyzed the physicochemical properties of two kinds of fish swim bladder pepsin-soluble collagen (PSC). Calculation of collagen yield, amino acid composition, SDS-PAGE, ultraviolet spectroscopy, Fourier infrared spectroscopy, circular dichroism, microdifferential scanning calorimeter to study the differences of the composition and physic-chemical properties of the two types of swim bladder PSC. The results showed that the content of crude fat and hydroxyproline in the basic nutrients of sturgeon swim bladder were significantly higher than that of grass carp swim bladder (P<0.05). The yield of sturgeon swim bladder was 80.63%, which was significantly higher than the PSC yield of grass carp swim bladder 49.85% (P<0.05).Two kinds of swim bladder PSC were consistent with type I collagen. The relative molecular weight of α chain and the content of dimer β chain of sturgeon swim bladder PSC were lower than those of grass carp swim bladder PSC. In the amino acid composition, the content of Ser, Met, Ile, Leu, His and Arg of sturgeon swim bladder PSC was significantly higher than that of grass carp swim bladder PSC (P<0.05), the content of Ala, Phe, Lys, Pro and Hyp of grass carp swim bladder PSC was significantly higher than that of sturgeon swim bladder PSC (P<0.05). They had similar secondary structures. The PSC denaturation temperature of sturgeon swim bladder was 29.26 ℃, which was significantly lower than that of the PSC of grass carp swim bladder, which was 36.01 ℃ (P<0.05). Among the two kinds of fish bladder PSC, grass carp swim bladder PSC had better thermal stability than sturgeon swim bladder PSC, but sturgeon swim bladder PSC had a higher yield than grass carp swim bladder PSC.

     

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