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中国精品科技期刊2020
周芷亦,何伟伟,李大婧,等. NaCl胁迫对西兰花芽苗叶黄素积累及抗氧化能力的影响[J]. 食品工业科技,2021,42(12):46−54. doi: 10.13386/j.issn1002-0306.2020100100.
引用本文: 周芷亦,何伟伟,李大婧,等. NaCl胁迫对西兰花芽苗叶黄素积累及抗氧化能力的影响[J]. 食品工业科技,2021,42(12):46−54. doi: 10.13386/j.issn1002-0306.2020100100.
ZHOU Zhiyi, HE Weiwei, LI Dajing, et al. Effects of NaCl Stress on Lutein Accumulation and Antioxidant Capacity of Broccoli Sprouts [J]. Science and Technology of Food Industry, 2021, 42(12): 46−54. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020100100.
Citation: ZHOU Zhiyi, HE Weiwei, LI Dajing, et al. Effects of NaCl Stress on Lutein Accumulation and Antioxidant Capacity of Broccoli Sprouts [J]. Science and Technology of Food Industry, 2021, 42(12): 46−54. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020100100.

NaCl胁迫对西兰花芽苗叶黄素积累及抗氧化能力的影响

Effects of NaCl Stress on Lutein Accumulation and Antioxidant Capacity of Broccoli Sprouts

  • 摘要: 为探究NaCl胁迫对西兰花芽苗叶黄素富集及抗氧化能力的影响,本实验以“青峰”西兰花种子为实验原材料,研究了不同浓度NaCl溶液处理对西兰花芽苗的芽长、鲜重、干重、含水率、可溶性蛋白、还原糖、丙二醛、脯氨酸、叶黄素含量及抗氧化酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响。结果表明,不同NaCl浓度对西兰花芽苗生长影响较大,随NaCl浓度由0~300 mmol/L变化,叶黄素含量先升高后下降。经100 mmol/L NaCl胁迫处理至第4 d收获的芽苗中叶黄素含量为546.25 μg/g,比对照组增加了37.54%,该条件下芽苗的芽长、鲜重、干重、含水率、可溶性蛋白、还原糖含量也均明显提高,较对照组分别增加了20.88%、161.16%、68.65%、2.86%、54.90%和40.0%,SOD、POD、CAT抗氧化酶活性与对照组相比分别增加了36.26%、14.62%、32.34%。本研究为通过NaCl胁迫西兰花种子萌发来提高叶黄素含量提供了新的方式,为开发功能性西兰花芽苗食品提供参考。

     

    Abstract: To explore the effect of NaCl stress on the lutein enrichment and antioxidant capacity of broccoli sprouts, the "Qingfeng" broccoli seeds were used as the experimental raw material. The sprouts length, fresh weight, dry weight, moisture content, soluble protein, reducing sugar, malondialdehyde, proline, lutein content and antioxidant enzymes including superoxide dismutase, peroxidase and peroxidase catalase activity were studied in this experiment. Results showed that different NaCl concentrations had a greater impact on the growth of broccoli sprouts. As NaCl concentration increased from 0 to 300 mmol/L, the content of lutein in the sprouts first increased and then decreased. Under 100 mmol/L NaCl stress, the lutein content in 4-day old sprouts was 546.25 g/g, which was 37.54% higher than that in the control group. The sprouts length, fresh weight, dry weight, moisture content, soluble protein and reducing sugar content also increased by 20.88%, 161.16%, 68.65%, 2.86%, 54.90% and 40.0%, respectively. At the same time, the antioxidant enzyme activities of SOD, POD and CAT increased by 36.26%, 14.62%, and 32.34% respectively. The results of this study provided a new way to improve lutein content through NaCl stress on the germination of broccoli seeds, thus providing a reference for the development of functional food of broccoli sprouts.

     

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