• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
付亚玲,姚俊修,张仁堂. 响应面法优化黑化红枣三萜酸提取工艺及抗氧化活性研究[J]. 食品工业科技,2021,42(12):176−183. doi: 10.13386/j.issn1002-0306.2020080218.
引用本文: 付亚玲,姚俊修,张仁堂. 响应面法优化黑化红枣三萜酸提取工艺及抗氧化活性研究[J]. 食品工业科技,2021,42(12):176−183. doi: 10.13386/j.issn1002-0306.2020080218.
FU Yaling, YAO Junxiu, ZHANG Rentang. Optimization of Extraction and Antioxidant Activities of Triterpenic Acids from Blacked Jujube by Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(12): 176−183. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080218.
Citation: FU Yaling, YAO Junxiu, ZHANG Rentang. Optimization of Extraction and Antioxidant Activities of Triterpenic Acids from Blacked Jujube by Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(12): 176−183. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080218.

响应面法优化黑化红枣三萜酸提取工艺及抗氧化活性研究

Optimization of Extraction and Antioxidant Activities of Triterpenic Acids from Blacked Jujube by Response Surface Methodology

  • 摘要: 目的:以黑化后的红枣为试材,研究其三萜酸提取工艺条件及抗氧化活性。方法:通过单因素实验和Box-Behnken响应面试验进行超声波提取黑化红枣三萜酸工艺优化,并测定黑化红枣三萜酸纯化前后对DPPH、ABTS自由基的清除能力和总还原力以评估其体外抗氧化活性。结果:黑化红枣中三萜酸的最优提取工艺参数为:50%乙醇浓度,液料比23:1 mL/g,超声时间30 min,超声功率300 W,在此条件下,三萜酸含量为1.313±0.01 mg/g;黑化红枣三萜酸粗提物和纯化物清除DPPH、ABTS自由基的半抑制浓度(IC50)分别为0.571、0.053和0.186、0.059 mg/mL,总还原力则与样品浓度呈现一定的量效关系。结论:该工艺简单、合理可行,黑化红枣三萜酸具有良好的抗氧化活性。

     

    Abstract: Objective: Blacked jujube was used as raw materials to study the extraction conditions and antioxidant activity of triterpenic acids. Methods: The ultrasonic extraction process of triterpenic acids from blacked jujube was optimized by single factor and Box-Behnken response surface experiments. The antioxidant activity of before and after purification of triterpenic acids were evaluated by determining the scavenging capacity of DPPH and ABTS+ free radicals and total reducing power. Results: The optimum extraction parameters were as follows: 50% ethanol concentration, liquid-solid ratio 23:1 mL/g, ultrasonic time 30 min, ultrasonic power 300 W. Under these conditions, the yield of triterpenoid acids was 1.313±0.01 mg/g. The half inhibitory concentration (IC50) for scavenging of DPPH· and ABTS+· of crude and purified triterpenoid acids from blacked jujube were 0.571, 0.053, 0.186 and 0.059 mg/mL, respectively. The total reducing power showed a certain dose effect relationship with the sample concentration. Conclusions: The process was simple, reasonable and feasible. The triterpenic acids of blacked jujube would have a good antioxidant activity.

     

/

返回文章
返回