• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
张嘉钰,辛嘉英,刘丰源,等. 甲烷氧化菌素-铜配合物模拟过氧化物酶检测面粉中的过氧化钙[J]. 食品工业科技,2021,42(9):257−262. doi: 10.13386/j.issn1002-0306.2020060120.
引用本文: 张嘉钰,辛嘉英,刘丰源,等. 甲烷氧化菌素-铜配合物模拟过氧化物酶检测面粉中的过氧化钙[J]. 食品工业科技,2021,42(9):257−262. doi: 10.13386/j.issn1002-0306.2020060120.
ZHANG Jiayu, XIN Jiaying, LIU Fengyuan, et al. Detection of Calcium Peroxide in Flour by Methanobactin-Cu Simulated Peroxidase Method[J]. Science and Technology of Food Industry, 2021, 42(9): 257−262. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020060120.
Citation: ZHANG Jiayu, XIN Jiaying, LIU Fengyuan, et al. Detection of Calcium Peroxide in Flour by Methanobactin-Cu Simulated Peroxidase Method[J]. Science and Technology of Food Industry, 2021, 42(9): 257−262. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020060120.

甲烷氧化菌素-铜配合物模拟过氧化物酶检测面粉中的过氧化钙

Detection of Calcium Peroxide in Flour by Methanobactin-Cu Simulated Peroxidase Method

  • 摘要: 通过紫外分光光度计检测CaO2在288 nm处吸光值的变化,建立了一种快速、准确的Mb-Cu模拟过氧化物酶法来检测面粉中的CaO2,并讨论了Mb-Cu浓度、反应温度、pH、反应时间对催化过程的影响。结果表明,当Mb-Cu浓度为3.9×10−6 mol/L、反应温度为60 ℃、反应时间为180 s时,CaO2在浓度为0~8 mg/L时与288 nm处吸光度的变化值呈线性关系,线性方程为y=0.00643+0.02117x,R2=0.99281。方法的检出限为1.82×10−2 mg/L,平均加标回收率为98.2%~100.6%,相对标准偏差RSD为0.49%~1.32%。此方法快速、准确,可用于对实际样品的测量。

     

    Abstract: By using the ultraviolet absorption spectrum to detect the change of absorption value of CaO2 at 288 nm, a fast and accurate method of CaO2 detecting in flour was established. The results showed that when the Mb-Cu concentration was 3.9×10−6 mol/L, the detection temperature was 60 ℃, and the detection time was 180 s, the CaO2 concentration was linear with the change in absorbance at 288 nm, and the linear equation was y=0.00643+0.02117x, R2=0.99281. The detection limit of the method was 1.82×10−2 mg/L, the average recovery of standard addition was 98.2%~100.6%, and the relative standard deviation RSD was 0.49%~1.32%. This Mb-Cu mimic peroxidase method has high accuracy, fast detection speed, and low detection limit. It can be used in practice for the trace detection of CaO2 in flour, providing a new strategy for the food detection industry.

     

/

返回文章
返回