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中国精品科技期刊2020
邱雪梅, 徐丽, 骆海龙, 袁杰, 杨靖, 李蓓, 汪静杰. 肺炎克雷伯菌对H2O2氧化胁迫的响应机制研究[J]. 食品工业科技, 2020, 41(21): 99-104. DOI: 10.13386/j.issn1002-0306.2020050357
引用本文: 邱雪梅, 徐丽, 骆海龙, 袁杰, 杨靖, 李蓓, 汪静杰. 肺炎克雷伯菌对H2O2氧化胁迫的响应机制研究[J]. 食品工业科技, 2020, 41(21): 99-104. DOI: 10.13386/j.issn1002-0306.2020050357
QIU Xue-mei, XU Li, LUO Hai-Long, YUAN Jie, YANG Jing, LI Bei, WANG Jing-jie. The Response Mechanism of Klebsiella pneumoniae to H2O2 Oxidative Stress[J]. Science and Technology of Food Industry, 2020, 41(21): 99-104. DOI: 10.13386/j.issn1002-0306.2020050357
Citation: QIU Xue-mei, XU Li, LUO Hai-Long, YUAN Jie, YANG Jing, LI Bei, WANG Jing-jie. The Response Mechanism of Klebsiella pneumoniae to H2O2 Oxidative Stress[J]. Science and Technology of Food Industry, 2020, 41(21): 99-104. DOI: 10.13386/j.issn1002-0306.2020050357

肺炎克雷伯菌对H2O2氧化胁迫的响应机制研究

The Response Mechanism of Klebsiella pneumoniae to H2O2 Oxidative Stress

  • 摘要: 目的:研究宿主肠道氧化条件对肺炎克雷伯菌的影响及该菌响应氧化胁迫的机制。方法:通过体外模拟肠道氧化条件,将肺炎克雷伯菌接种在含不同浓度梯度H2O2的液体培养基中进行氧化胁迫处理;测定细菌的生长曲线、检测细菌的关键毒力因子-荚膜与生物膜的生成量。并进一步地利用qRT-PCR荧光定量分析荚膜与生物膜表型相关基因的表达差异。结果:在氧化因子胁迫下,当处于较低浓度范围(<1.56 mmol/L)时,肺炎克雷伯菌早期生长受到抑制作用,中后期逐渐适应胁迫、其生长未受明显影响。此外,肺炎克雷伯菌在氧化胁迫条件下荚膜生成量明显降低,呈剂量依赖效应;但在一定浓度范围氧化因子作用下,其生物膜的形成量增加。分子检测表明荚膜结构基因magA表达量明显下调,生物膜相关基因YbaJ出现显著的上调表达。结论:肺炎克雷伯菌通过上调YbaJ基因的表达促进毒力因子生物膜形成以此响应氧化因子胁迫,本研究可为食源性肺炎克雷伯菌与宿主肠道因子互作及致病机制研究提供新思路。

     

    Abstract: Objective:This study aimed at revealing the effect of host intestinal oxidative condition on Klebsiella pneumoniae and its mechanism of response to oxidative stress.Method:In vitro intestinal oxidative conditions,Klebsiella pneumoniae was inoculated in liquid medium containing different concentration of H2O2 for oxidative stress treatment,the growth curve of bacteria was measured,and the key virulence factors of bacteria--capsule and biofilm were examined. Furthermore,qRT-PCR quantitative analysis was used to analyze the differences in the expression of genes related to the phenotype of the capsule and biofilm. Results:The results showed that under condition of H2O2 oxidative stress,when it was in a lower concentration range(<1.56 mmol/L),the early growth stage of Klebsiella pneumoniae was inhibited,and it gradually adapted to stress in the middle and late stages,and the growth was not significantly affected. The production of Klebsiella pneumoniae capsule was significantly reduced with a dose-dependent effect;while under a certain concentration range of oxidative factors,the amount of biofilm formation increased. And molecular testing showed that the expression level of the capsular structure gene magA was significantly down-regulated,and the biofilm-related gene YbaJ was significantly up-regulated. Conclusions:Taken the results together,Klebsiella pneumoniae could up-regulate the expression of gene YbaJ to promote the formation of virulence factor biofilm in response to oxidative factor stress,which provided new ideas for the study of the interaction between Klebsiella pneumoniae and host intestinal factors and the pathogenic mechanism.

     

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