亚基水平上大豆蛋白凝胶性的研究进展

付洪玲 范宏亮 吕博 孙贺 单单单 于寒松

付洪玲,范宏亮,吕博,等. 亚基水平上大豆蛋白凝胶性的研究进展[J]. 食品工业科技,2021,42(7):382−389. doi: 10.13386/j.issn1002-0306.2020050338
引用本文: 付洪玲,范宏亮,吕博,等. 亚基水平上大豆蛋白凝胶性的研究进展[J]. 食品工业科技,2021,42(7):382−389. doi: 10.13386/ j.issn1002-0306.2020050338
FU Hongling, FAN Hongliang, LYU Bo, et al. Research Progress of Soybean Protein Gels at Subunit Level[J]. Science and Technology of Food Industry, 2021, 42(7): 382−389. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020050338
Citation: FU Hongling, FAN Hongliang, LYU Bo, et al. Research Progress of Soybean Protein Gels at Subunit Level[J]. Science and Technology of Food Industry, 2021, 42(7): 382−389. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020050338

亚基水平上大豆蛋白凝胶性的研究进展

doi: 10.13386/j.issn1002-0306.2020050338
基金项目: 国家大豆产业技术体系(CARS-04)
详细信息
    作者简介:

    付洪玲(1995−),女,硕士研究生,研究方向:大豆分离蛋白加工性能研究,E-mail:15764381475@163.com

    通讯作者:

    于寒松(1979−),男,博士,教授,研究方向:大豆精深加工及综合利用,E-mail:yuhansong@163.com

  • 中图分类号: TS201.7

Research Progress of Soybean Protein Gels at Subunit Level

  • 摘要: 大豆蛋白主要由β-伴大豆球蛋白(7S球蛋白)和大豆球蛋白(11S球蛋白)组成,不同亚基组成直接影响着大豆蛋白的加工特性。凝胶特性是本研究的主题。本文总结了大豆蛋白的组成及其化学结构,介绍7S球蛋白和11S球蛋白的凝胶形成过程,同时对亚基组分对凝胶性的影响和蛋白亚基缺失型大豆的研究进展进行概述,为大豆蛋白产品的开发及应用提供重要的理论指导。
  • 图  1  β-伴大豆球蛋白及其六个异构体的亚基结构示意图[23]

    Figure  1.  Schematic representation of the subunit structure of β-conglycinin and its six isomers[23]

    图  2  大豆蛋白晶体结构图[26,28]

    注:a:天然β-同源三聚体的带状模型;b:大豆球蛋白A1aB1b同源六聚体的晶体结构;c:大豆球蛋白A3B4同源六聚体的晶体结构。

    Figure  2.  The crystal structure of soy protein [26,28]

    图  3  大豆球蛋白凝胶化过程中可溶性聚集体的形成[39]

    Figure  3.  Formation of soluble aggregates during the gelation of glycinin[39]

    图  4  在pH7.0下7S球蛋白和11S球蛋白的热聚集行为的图解描述[4142]

    注:N: 天然状态; U: 展开状态; Agg: 聚集状态。

    Figure  4.  Diagrammatic depiction of thermal aggregation behavior of β-conglycinin and glycinin at pH 7.0 [4142]

    表  1  亚基缺失型大豆品种

    Table  1.   Subunit- deficiency soybean varieties

    研究者方法缺失类型
    国外Kitamura等[62]SDS-PAGE法α′亚基缺失型和α亚基、β亚基含量较少
    Takahashi等[63]γ射线辐射技术α+α′)亚基缺失,β亚基含量较低
    Odanaka等[64]γ射线辐射技术11S球蛋白I组亚基缺失
    国内郭顺堂等[65]SDS-PAGE及Western Blot法首次在中国大豆品种中发现7S球蛋白的β-亚基缺失
    宋波等[66]杂交育种具有中国遗传背景的α′亚基缺失型、α亚基缺失型、(α′+α)亚基缺失型
    韩艳婧[67]回交转育法具有中国大豆遗传背景的且具有低致敏性的α′亚基缺失以及近等基因系
    张亚琴等[68]生物信息学分析及亚细胞定位A1aB1b亚基自然缺失型-桂阳紫金豆
    李俊英等[69]SDS-PAGE及Western Blot法α′亚基缺失突变体-中黄608大豆品种
    下载: 导出CSV
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