响应面优化紫果西番莲叶多酚超声辅助提取工艺及其抗氧化活性

贺银菊; 张旋俊; 杨再波; 彭莘媚; 卯申发

doi:10.13386/j.issn1002-0306.2020030146

响应面优化紫果西番莲叶多酚超声辅助提取工艺及其抗氧化活性

doi: 10.13386/j.issn1002-0306.2020030146

1. 黔南民族师范学院化学化工学院, 贵州都匀 558000;
2. 贵州省高校民族药用植物资源开发工程研究中心, 贵州都匀 558000

基金项目:

黔南州科技局计划项目（黔南科合社字（2016）41号）；校级产学研基地（Qnsyk201603，QNSYCXYJD2018005）；校级大学生科技创新创业训练项目（QNSY2019DKC004）。

详细信息

作者简介:
贺银菊(1986-),女,硕士,讲师,主要从事天然产物的研究与开发方面的研究,E-mail:heyinju2007@163.com。

中图分类号: TS201.1
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出版历程
- 收稿日期: 2020-03-13

Optimization of Ultrasonic-Assisted Extraction of Polyphenols in the Purple Passionfruit Leaves by Response Surface Methodology and Its Antioxidant Activity

1. School of Chemistry and Chemical Engineering, Qiannan Normal University for Nationalities, Duyun 558000, China;
2. Guizhou Province College Research Center of Ethnical Medicinal Plant Resources Ex-ploitation Engineering, Duyun 558000, China

摘要

摘要: 以紫果西番莲叶为对象，研究其多酚提取工艺及抗氧化活性。在单因素实验基础上采用Box-Behnken响应面分析法优化紫果西番莲叶多酚的提取工艺，考察液料比、提取时间、超声功率和超声温度对其多酚提取量的影响，以清除DPPH自由基和·OH能力评价紫果西番莲叶多酚的抗氧化活性。结果表明，最佳提取条件为：液料比36：1 mL/g、提取时间54 min、超声功率350 W和超声温度70℃，此时紫果西番莲叶中多酚提取量为（13.19±0.17） mg/g。抗氧化活性结果表明，紫果西番莲叶多酚具有较好的抗氧化活性，其清除DPPH自由基和·OH的半抑制浓度（IC₅₀）分别为0.058和0.144 mg/mL。
- 紫果西番莲叶 /
- 响应面分析 /
- 多酚 /
- 提取 /
- 抗氧化活性
Abstract: To optimize the extraction technology and antioxidant activity of polyphenols from purple passionfruit leaves. On the basis of a single factor test,the material to solvent ratio,extraction time,ultrasonic power and ultrasonic temperature were the factors,and the Box-Beheken was used to design test program. The optimal extraction technology was as follows: Solvent to material ratio 36∶1 mL/g,extraction time 54 min,ultrasonic power 350 W and temperature 70 ℃. Under the above conditions,the extraction amount of polyphenols from purple passionfruit leaves was(13.19±0.17) mg/g. The test of antioxidant activity showed that the polyphenols from purple passionfruit leaves had better antioxidant activity,and half inhibition concentrations(IC₅₀)of DPPH and hydroxyl radicals were 0.058 mg/mL and 0.144 mg/mL,respectively.
- purple passionfruit leaves /
- response surface methodology /
- polyphenols /
- extraction /
- antioxidant activity

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