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中国精品科技期刊2020
毕萃萃, 刘银路, 杨丽涛, 魏芬芬, 李勇超, 张波. 雨生红球藻破壁孢子粉对Ⅱ型糖尿病大鼠肝肾的保护作用[J]. 食品工业科技, 2021, 42(1): 328-333. DOI: 10.13386/j.issn1002-0306.2020020325
引用本文: 毕萃萃, 刘银路, 杨丽涛, 魏芬芬, 李勇超, 张波. 雨生红球藻破壁孢子粉对Ⅱ型糖尿病大鼠肝肾的保护作用[J]. 食品工业科技, 2021, 42(1): 328-333. DOI: 10.13386/j.issn1002-0306.2020020325
BI Cui-cui, LIU Yin-lu, YANG Li-tao, WEI Fen-fen, LI Yong-chao, ZHANG Bo. Protective Effects of Sporoderm-broken Spore Powder of Haematococcus pluvialis on Liver and Kidney of Type 2 Diabetes Rats[J]. Science and Technology of Food Industry, 2021, 42(1): 328-333. DOI: 10.13386/j.issn1002-0306.2020020325
Citation: BI Cui-cui, LIU Yin-lu, YANG Li-tao, WEI Fen-fen, LI Yong-chao, ZHANG Bo. Protective Effects of Sporoderm-broken Spore Powder of Haematococcus pluvialis on Liver and Kidney of Type 2 Diabetes Rats[J]. Science and Technology of Food Industry, 2021, 42(1): 328-333. DOI: 10.13386/j.issn1002-0306.2020020325

雨生红球藻破壁孢子粉对Ⅱ型糖尿病大鼠肝肾的保护作用

Protective Effects of Sporoderm-broken Spore Powder of Haematococcus pluvialis on Liver and Kidney of Type 2 Diabetes Rats

  • 摘要: 目的:研究雨生红球藻破壁孢子粉对Ⅱ型糖尿病大鼠肝肾的保护作用。方法:将SD大鼠随机分为正常组和造模组,正常组以普通饲料喂养,造模组70只大鼠采用高脂高糖饲料饲养4周后,腹腔注射一次链脲佐菌素(STZ)35 mg/kg BW;继续饲养4周,测血糖将造模成功大鼠再分为模型组、低剂量组、中剂量组、高剂量组、降糖药物组(模型组灌胃蒸馏水,低、中、高三个剂量组分别给予50、100和200 mg/kg BW的雨生红球藻破壁孢子粉,降糖药物组给予28.5 mg/kg BW的盐酸二甲双胍),连续干预4周,处死大鼠;测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)活性以及白蛋白(ALB)和球蛋白(GLO)含量,测定肝肾组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量以及肿瘤坏死因子(TNF-α)、白介素1β(IL-1β)水平;制作肝肾HE染色病理切片,观察病理学变化。结果:高剂量雨生红球藻破壁孢子粉可极显著降低糖尿病大鼠血清ALT、AST、ALP、LDH活性和GLO含量(P<0.01),显著减少ALB含量(P<0.05);极显著提高肝肾组织中SOD和GSH-Px活性(P<0.01),极显著降低MDA含量(P<0.01);极显著降低肝肾组织TNF-α和IL-1β水平(P<0.01)。雨生红球藻破壁孢子粉对肝肾组织的损伤有一定的缓解作用。结论:雨生红球藻破壁孢子粉可减轻糖尿病大鼠的肝肾损伤,机制可能与降低氧化应激和炎性反应有关。

     

    Abstract: Objective: To study the protective effect of sporoderm-broken spore powder of Haematococcus pluvialis on liver and kidney in type 2 diabetes rats. Methods: SD rats were divided into normal group and model group. The normal group was fed with ordinary feed,and 70 rats were modeled with high-fat and high-sugar diet for 4 weeks. Then intraperitoneal injection of streptozotocin 35 mg/kg BW;continued feeding for 4 weeks,and the blood glucose was measured to divide the successful model rats into model group,low dose group,medium dose group,high dose group,and hypoglycemic drug group. The rats in model group were administrated with double distilled water,meanwhile,the low,medium,and high dose groups were administered orally to 50,100 and 200 mg/kg BW,respectively,the rats in hypoglycemic drug group were administered orally with metformin hydrochloride(28.5 mg/kg BW),and continued to be fed with high-fat and high-sugar feed for 4 weeks. The rats were sacrificed. The alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),lactate dehydrogenase(LDH)activities,albumin(ALB)and globulin(GLO)contents of the serum were determined. Liver and kidney were obtained for determining the related markers,including superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),and the inflammatory factor levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β).Liver and kidney HE stained pathological sections were made to observe the liver and kidney histopathological changes. Results: Compared with the model group,rats in treatment groups had a significance reduction in serum ALT,AST,ALP,LDH activities,GLO content(P<0.01)and ALB content(P<0.05)in type 2 diabetes rats. The treatment groups significantly increased the activity of SOD and GSH-Px in liver and kidney tissues(P<0.01),reduce the content of MDA(P<0.01),and significantly reduce the levels of TNF-α and IL-1β(P<0.01). Furthermore,the pathological injury of liver and kidney tissues were also significantly improved. Conclusion: Sporoderm-broken spore powder of Haematococcus pluvialis can ameliorate the liver and kidney damage in type 2 diabetes rats. The mechanism may be related to reducing oxidative stress and reducing inflammatory responses.

     

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