pH响应比色酶联免疫吸附法检测牛奶中猪霍乱沙门氏菌

李倩影 张抗抗 周耀锋 黄小林 李响敏 熊勇华

李倩影, 张抗抗, 周耀锋, 黄小林, 李响敏, 熊勇华. pH响应比色酶联免疫吸附法检测牛奶中猪霍乱沙门氏菌[J]. 食品工业科技, 2021, 42(1): 227-232. doi: 10.13386/j.issn1002-0306.2019100100
引用本文: 李倩影, 张抗抗, 周耀锋, 黄小林, 李响敏, 熊勇华. pH响应比色酶联免疫吸附法检测牛奶中猪霍乱沙门氏菌[J]. 食品工业科技, 2021, 42(1): 227-232. doi: 10.13386/j.issn1002-0306.2019100100
LI Qian-ying, ZHANG Kang-kang, ZHOU Yao-feng, HUANG Xiao-lin, LI Xiang-min, XIONG Yong-hua. pH Responded Colorimetric ELISA for Sensitive Detection of Salmonella Choleraesuis in Milk[J]. Science and Technology of Food Industry, 2021, 42(1): 227-232. doi: 10.13386/j.issn1002-0306.2019100100
Citation: LI Qian-ying, ZHANG Kang-kang, ZHOU Yao-feng, HUANG Xiao-lin, LI Xiang-min, XIONG Yong-hua. pH Responded Colorimetric ELISA for Sensitive Detection of Salmonella Choleraesuis in Milk[J]. Science and Technology of Food Industry, 2021, 42(1): 227-232. doi: 10.13386/j.issn1002-0306.2019100100

pH响应比色酶联免疫吸附法检测牛奶中猪霍乱沙门氏菌

doi: 10.13386/j.issn1002-0306.2019100100
基金项目: 

"十三五"国家重点研发计划(2018YFC1602202、2018YFC1602203、2018YFC1602505);国家自然科学基金(31760485、31901780)。

详细信息
    作者简介:

    李倩影(2000-),女,学士,研究方向:食品安全快速检测,E-mail:1464561886@qq.com;张抗抗(1998-),女,学士,研究方向:食品安全快速检测,E-mail:m15797899516@163.com。

    通讯作者:

    李响敏(1973-),男,博士,讲师,研究方向:食品质量与控制,E-mail:lixiangmin73@163.com。

  • 中图分类号: TS207.4

pH Responded Colorimetric ELISA for Sensitive Detection of Salmonella Choleraesuis in Milk

  • 摘要: 人类常常因误食被猪霍乱沙门氏菌感染的食物而引发中毒。因此,急需构建适用于快速、灵敏检测食物中猪霍乱沙门氏菌污染的新方法。鉴于此,本文构建了一种pH响应比色酶联免疫吸附法灵敏检测牛奶中猪霍乱沙门氏菌。该方法利用葡萄糖氧化酶催化底物葡萄糖产生葡萄糖酸,导致溶液pH下降,进而引发溴甲酚紫(Bromocresol purple,BCP)溶液颜色由紫色变成亮黄色,随后通过记录BCP颜色变化(OD430/OD590)实现目标菌的定量检测。当菌浓度为2.54×103~6.17×105 CFU/mL,该方法定量检测猪霍乱沙门氏菌可用方程一表述:y1=0.1051ln (x)+0.7024(R2=0.7513);当菌浓度为6.17×105~1.67×107 CFU/mL,该方法定量检测猪霍乱沙门氏菌可用方程二表述:y2=1.3216ln (x)-15.797(R2=0.9711);当菌浓度大于1.67×107 CFU/mL时,BCP溶液呈现明显亮黄色,OD430/OD590值趋于稳定,无法实现猪霍乱沙门氏菌定量检测。将六个不同浓度的猪霍乱沙门氏菌(2.5×103~5.6×106 CFU/mL)加标至牛奶中,检测结果显示回收率介于72.16%~103.58%,相对标准偏差介于7.54%~15.30%。总之,本研究所构建的比色ELISA方法适用于牛奶中不同浓度的猪霍乱沙门氏菌快速、灵敏定量检测。
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  • 收稿日期:  2019-10-16

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