地衣芽孢杆菌总蛋白双向电泳方法的建立和优化

地衣芽孢杆菌总蛋白双向电泳方法的建立和优化[J]. 食品工业科技, 2013, (15): 170-173. doi: 10.13386/j.issn1002-0306.2013.15.061
引用本文: 地衣芽孢杆菌总蛋白双向电泳方法的建立和优化[J]. 食品工业科技, 2013, (15): 170-173. doi: 10.13386/j.issn1002-0306.2013.15.061
Establishment and optimization of two-dimensional gel electrophoresis of total proteins from Bacillus lincheniformis[J]. Science and Technology of Food Industry, 2013, (15): 170-173. doi: 10.13386/j.issn1002-0306.2013.15.061
Citation: Establishment and optimization of two-dimensional gel electrophoresis of total proteins from Bacillus lincheniformis[J]. Science and Technology of Food Industry, 2013, (15): 170-173. doi: 10.13386/j.issn1002-0306.2013.15.061

地衣芽孢杆菌总蛋白双向电泳方法的建立和优化

doi: 10.13386/j.issn1002-0306.2013.15.061
基金项目: 

九江学院博士启动基金(8871009);

详细信息
  • 中图分类号: TS201.2

Establishment and optimization of two-dimensional gel electrophoresis of total proteins from Bacillus lincheniformis

  • 摘要: 探索建立有效的地衣芽孢杆菌蛋白质组双向电泳体系,为进一步揭示地衣芽孢杆菌促进氧化葡萄糖酸杆菌产酸的作用机制奠定基础。以地衣芽孢杆菌为材料,比较蛋白质制备超声破壁时间、新型细胞裂解液、pH梯度和不同上样量对地衣芽孢杆菌蛋白双向电泳结果的影响。结果显示:采用15min超声破壁提取地衣芽孢杆菌总蛋白,选用新型蛋白质裂解,用长24cm、pH4~7的IPG胶条,在上样量为80μg进行等电聚焦,于60V15min、120V6h条件下进行SDS-PAGE垂直电泳,可以获得背景清晰、重复性好的双向电泳图谱。在探索出一种新型可行的新型细胞裂解液的同时,建立一套用于地衣芽孢杆菌蛋白质组分析的双向电泳方法。 
  • [1] Gallie DR.The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth[J].Journal of Experimental Botany, 2013, 64 (2) :433-443.
    [2] 赵军, 康艳红, 杨伟超, 等.葡萄糖对维生素C二步发酵法生产2-酮基-L-古龙酸影响的研究[J].新疆农业大学学报, 2008, 31 (4) :50-53.
    [3] 宋文新, 邵庆均.维生素C二步发酵合成法的研究进展[J].中国饲料, 2008 (19) :9-13.
    [4] Luo Y, Vilain S, Voigt B, et al.Proteomic analysis of Bacillus cereus growing in liquid soil organic matter[J].Journal Fems Microbiology Letters, 2007, 271 (1) :40-47.
    [5] Voigt B, Schweder T, Becher D, et al.A proteomic view of cell physiology of Bacillus licheniformis[J].Journal Proteomics, 2004, 4 (5) :1465-1490.
    [6] 吕淑霞, 周丽娜, 冯树, 等.巨大芽孢杆菌在维生素C二步发酵中的作用[J].微生物学杂志, 2001, 21 (3) :3-5.
    [7] Shujuan L, Jun W, Jiangming Yao, et al.Study on the Effect of Mutated Bacillus Megaterium in Two-Stage Fermentation of Vitamin C[J].Journal Plasma Science and Technology, 2003, 5 (5) :2011-2016.
    [8] Wilkins MR, Sanchez JC, Gooley AA, et al.Progress with proteome projects:why all proteins expressed by a genome shouldbe[J].Advances in Biochemical Engineering/Biotechnology, 1996 (13) :19-50.
    [9] 刘频健, 陈静, 姜登钊, 等.蜡样芽孢杆菌总蛋白双向电泳方法的建立和优化[J].军事医学, 2012, 36 (12) :942-945.
    [10] Anderson I, Sorokin A, Kapatral V, et al.Comparative genome analysis of Bacillus cereus group genomes with Bacillus subtilis[J].Journal Fems Microbiology Letters, 2005, 250 (2) :175-184.
    [11] Rey MW, Ramaiya P, Nelson BA, et al.Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species[J].Journal Genome Biology, 2004, 5 (10) :R77.
    [12] 孔令琼, 管政兵, 陆健, 等.绍兴黄酒成品麦曲中微生物胞外酶的双向电泳技术的建立[J].食品与生物技术学报, 2011, 30 (3) :453-457.
    [13] 张小果, 乔桂荣, 李翠云, 等.旱柳根系蛋白质双向电泳体系的建立[J].浙江农林大学学报, 2011, 28 (4) :653-661.
    [14] 张容鹄, 冯建成, 彭超, 等.桶形芋螺毒管蛋白质组双向电泳条件优化[J].生物技术, 2010, 20 (3) :60-62.
    [15] 梁文裕, 王星, 焦广飞, 等.发菜蛋白质组双向电泳技术的建立及优化[J].西北植物学报, 2009, 29 (8) :1550-1556.
    [16] Martínez-Gomariz M, Hernáez ML, Gutiérrez D, et al.Proteomic analysis by two-dimensional differential gel electrophoresis (2D DIGE) of a high-pressure effect in Bacillus cereus[J].Journal of Agricultural and Food Chemistry, 2009, 57 (9) :3543-3549.
  • 加载中
计量
  • 文章访问数:  76
  • HTML全文浏览量:  10
  • PDF下载量:  213
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-12-10

目录

    /

    返回文章
    返回

    重要通知

    《食品工业科技》喊你参加论坛啦:第五届食品科技创新论坛期待你的加入。