Inclusion complex of volatile oil from Thymus mongolicus Ronn.with hydroxypropyl-β-cyclodextrin
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摘要: 采用正交实验与综合评分相结合的方法优化水溶液搅拌法制备百里香挥发油-羟丙基-β-环糊精（HP-β-CD）包合物的工艺条件,运用傅立叶变换红外光谱（FT-IR）对包合物进行表征,并利用气相色谱-质谱联用（GC-MS）技术对比分析了包合前后百里香挥发油的化学成分。结果表明,水溶液搅拌法制备百里香挥发油HP-β-CD包合物的最佳工艺条件为:百里香挥发油与HP-β-CD的体积质量比为1:10（mL:g）,包合温度30℃,搅拌时间2.5h,HP-β-CD的浓度5%,在此最佳工艺下,平均包合物得率与挥发油利用率分别为91.77%和81.94%,该工艺合理、稳定、可行,有效地提高了百里香挥发油的稳定性。FT-IR和GC-MS分析说明,百里香挥发油与HP-β-CD已经形成了包合物,包合前后百里香挥发油的主要化学成分基本相同,但挥发油中各组分的组成比例发生了一定程度的改变,包合对百里香挥发油的化学成分略有影响。
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关键词:
- 百里香挥发油; /
- 羟丙基-β-环糊精; /
- 包合作用; /
- 化学成分;
Abstract: The orthogonal design and comprehensive evaluation methods were used to optimize the hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion technology of volatile oil from Thymus mongolicus and the aqueous solution stirring method was used to prepare the inclusion complex.The inclusion complex was characterized by fourier transform infrared (FT-IR) spectrum and the main chemical components change of volatile oil before and after being included by HP-β-CD were compared and analysed through gas chromatography-mass spectrometer (GC-MS) .The results showed that the optimal inclusion technology conditions were as follows:the ratio of volatile oil to HP-β-CD was 1:10 (mL:g) , the inclusion temperature and time was 30℃ for 2.5h, the concentration of HP-β-CD was 5%.The yield of inclusion complex and the utilization efficiency of volatile oil were 91.77% and 81.94% respectively under this optimal condition.The optimal inclusion technology conditions were reasonable, stable and feasible, the stabilities of the volatile oil were increased after being included by HP-β-CD.FT-IR and GC-MS analysis showed that the thymus volatile oil-HP-β-CD inclusion complex exhibitized some new physical characteristics and this mean the inclusion complex had been formed, the main chemical components of thymus volatile oil did not changed before and after being included by HP-β-CD, but the proportion of the components had been changed.HP-β-CD inclusion had slight effect on the chemical components and their proportion of the thymus volatile oil. -
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