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高效液相色谱-荧光法测定腊肉中的苯并芘残留

高效液相色谱-荧光法测定腊肉中的苯并芘残留[J]. 食品工业科技, 2013, (01): 319-322. doi: 10.13386/j.issn1002-0306.2013.01.022
引用本文: 高效液相色谱-荧光法测定腊肉中的苯并芘残留[J]. 食品工业科技, 2013, (01): 319-322. doi: 10.13386/j.issn1002-0306.2013.01.022
Determination of Benzo (a) pyrene in preserved ham by high performance liquid chromatography-fluorescence detection[J]. Science and Technology of Food Industry, 2013, (01): 319-322. doi: 10.13386/j.issn1002-0306.2013.01.022
Citation: Determination of Benzo (a) pyrene in preserved ham by high performance liquid chromatography-fluorescence detection[J]. Science and Technology of Food Industry, 2013, (01): 319-322. doi: 10.13386/j.issn1002-0306.2013.01.022

高效液相色谱-荧光法测定腊肉中的苯并芘残留

doi: 10.13386/j.issn1002-0306.2013.01.022
基金项目: 

国家公益性行业(农业)项目(200903012);

详细信息
  • 中图分类号: TS251.7;O657.31

Determination of Benzo (a) pyrene in preserved ham by high performance liquid chromatography-fluorescence detection

  • 摘要: 建立高效液相色谱-荧光法检测腊肉中苯并芘的分析方法。以市售的腊肉作为供试样品,样品经皂化,正己烷超声提取后,用WatersXBridgeTMC18柱分离,甲醇和水(90:10,v/v)为流动相,流速为1.0mL/min,荧光检测器激发波长为365nm,检测波长为410nm,外标法峰面积定量。结果表明:苯并芘在0.002~0.5μg/mL范围内呈现良好的线性关系,相关系数0.9999,在1~10μg/kg添加水平时,加标回收率为85.14%~91.01%,相对标准偏差为1.96%~5.37%(n=6);方法的检出限和定量限分别为0.15和0.5μg/kg。该方法测定腊肉中的苯并芘方便,快捷,准确可靠,易推广使用。 
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出版历程
  • 收稿日期:  2012-07-16

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