木芙蓉（Hibiscus mutabilis）叶提取物对大肠杆菌O1和金黄色葡萄球菌91053的体外抗菌效果研究
doi: 10.13386/j.issn1002-0306.2013.01.005
详细信息Antibacterial effect of extraction from Hibiscus mutabilis leaf on E.coli O1 and Staphylococcus aureus 91053
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摘要: 研究不同浓度木芙蓉(Hibiscus mutabilis)叶提取物对大肠杆菌(E.coliO1)和金黄色葡萄球菌(Staphylococcus aureus91053)的体外抗菌效果。利用二倍稀释法和涂布平板法,确定提取物对两种菌种的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),并绘制杀菌曲线。结果为:提取物对大肠杆菌的MIC和MBC浓度均为0.25g/mL,8、4、2、1MIC浓度的木芙蓉叶提取物分别在第6、14、18h能完全杀灭细菌该菌;提取物对金黄色葡萄球菌的MIC和MBC浓度均为0.5g/mL,4、2、1MIC浓度的木芙蓉叶提取物对金黄色葡萄球菌的杀菌效果更佳,分别能在第6、10、14h完全杀灭该菌。木芙蓉叶提取物对大肠杆菌和金黄色葡萄球菌均具明显的杀菌效果。Abstract: Antibacterial effect of extraction from Hibiscus mutabilis leaf on E.coli O1 and Staphylococcus aureus 91053 were studied, respectively.With the double broth dilution methods and spread plate methods, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract on E.coli O1 and Staphylococcus aureus 91053 were determined to draw antibacterial curves.The results showed that MIC and MBC of the extraction for E.coli O1 were both 0.25 g/mL, 8, 4, 2 and 1 times MIC could completely kill the test strain at 6, 14, 18 h, respectively;MIC and MBC of the extract on Staphylococcus aureus were both 0.5g/mL, 4, 2 and 1 times MIC of the extraction had better effect and could thoroughly kill the test strain at 8, 12 and 16h, respectively.The extraction could be bactericidal to the two test samples efficiently.
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