Study on angiotensin converting enzyme inhibition activity and active ingredient of traditional Chinese medicines
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摘要: 比较32味中药对血管紧张素转化酶（angiotensin converting enzyme,ACE）的抑制作用,并对枸杞的活性成分进行鉴别。高效液相色谱法（HPLC）评价药物对ACE活力的抑制作用,柱层析法对具有最强抑制作用的中药进行分离,确定活性最高的部位。32味中药提取物对ACE有不同程度的抑制作用,其中抑制作用最强的是枸杞,抑制率高达92.30%。枸杞提取物用D-101大孔树脂粗分,50%乙醇洗脱部位对ACE的抑制活性最强,此部位通过硅胶柱进一步纯化得到纯度更高的ACE抑制部位及活性成分。枸杞提取物对ACE酶有很强的抑制效果,大孔树脂和硅胶能很好的分离枸杞中对ACE有抑制作用的化合物。Abstract: Evaluation the angiotensin converting enzyme (ACE) inhibitory activity of 32 kinds of traditional Chinese medicines, and identified the active ingredient of Lycium barbarum L.ACE inhibition activity of traditional Chinese medicine was evaluated by high performance liquid chromatography (HPLC) method.The herb extract, which showed the highest ACE inhibitory activity, was further separated by column chromatography and the ACE inhibitory activity of each fraction was compared.Most herbs showed ACE inhibitory activity.Among them, Lycium barbarum L.exhibited the highest inhibitory rate (92.30%) .The Lycium barbarum L.extracts were separated using D-101 macroporous adsorption resins and the fraction which eluted by 50% ethyl alcohol exhibited the maximal inhibitory effect.The 50% ethyl alcohol fraction was separated using silica gel column, which gained the fraction and active ingredient of higher inhibitory effect.In 32 herbs, Lycium barbarum L.showed the highest ACE inhibitory activity.Besides, macroporous resin and silica gel could be used for pre-separation of Lycium barbarum L.extracts.
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