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菊糖果糖转移酶基因在毕赤酵母中的分泌表达

菊糖果糖转移酶基因在毕赤酵母中的分泌表达[J]. 食品工业科技, 2013, (01): 171-174. doi: 10.13386/j.issn1002-0306.2013.01.001
引用本文: 菊糖果糖转移酶基因在毕赤酵母中的分泌表达[J]. 食品工业科技, 2013, (01): 171-174. doi: 10.13386/j.issn1002-0306.2013.01.001
Secretory expression of inulin fructotransferase in Pichia pastoris[J]. Science and Technology of Food Industry, 2013, (01): 171-174. doi: 10.13386/j.issn1002-0306.2013.01.001
Citation: Secretory expression of inulin fructotransferase in Pichia pastoris[J]. Science and Technology of Food Industry, 2013, (01): 171-174. doi: 10.13386/j.issn1002-0306.2013.01.001

菊糖果糖转移酶基因在毕赤酵母中的分泌表达

doi: 10.13386/j.issn1002-0306.2013.01.001
详细信息
  • 中图分类号: Q786

Secretory expression of inulin fructotransferase in Pichia pastoris

  • 摘要: 将PCR获得的不包含信号肽序列的菊糖果糖转移酶基因(ift)与毕赤酵母分泌表达载体pPIC9K进行连接,构建重组载体pPIC9K-IFTase。重组载体经线性化后电转入毕赤酵母GS115感受态细胞,利用G418抗性梯度筛选及PCR鉴定,获得一株高拷贝菊糖果糖转移酶重组毕赤酵母菌株。该重组菌株经甲醇诱导,能够分泌具有活性的菊糖果糖转移酶,且60h时酶活为10.3U/mL。结果表明,菊糖果糖转移酶可以实现在毕赤酵母的分泌表达。 
  • [1] Tamura A, Nino H, Minobe T, et al.Difructose anhydride IIIdoes not contribute to body energy accumulation in rats[J].Bioscience, Biotechnology and Biochemistry, 2006, 70 (6) :1416-1422.
    [2] Saito K, Tomita F.Difructose anhydrides:Their mass-production and physiological functions[J].Bioscience, Biotechnology and Biochemistry, 2000, 64 (7) :1321-1327.
    [3] Minamida K, Asakawa C, Sujaya IN, et al.Effects of long-term ingestion of difructose anhydride III (DFAⅢ) on intestinal bacteria and bile acid metabolism in humans[J].Journal of Bioscience and Bioengineering, 2006, 101 (2) :149-156.
    [4] Minamida K, Ohashi M, Hara H, et al.Effects of ingestion of difructose anhydride III (DFAⅢ) and the DFAⅢ-assimilating bacterium Ruminococcus productus on rat intestine[J].Bioscience Biotechnology and Biochemistry, 2006, 70 (2) :332-339.
    [5] Shiga K, Nishimukai M, Tomita F, et al.Ingestion of difructose anhydride III, a non-digestible disaccharide, prevents gastrectomy-induced iron malabsorption and anemia in rats[J].Nutrition, 2006, 22 (7-8) :786-793.
    [6] 赵萌.菊糖果糖转移酶的菌种筛选、诱导合成、分离纯化及克隆表达[D].无锡:江南大学, 2012.
    [7] Haraguchi K, Mori S, Hayashi K.Cloning of inulin fructotransferase (DFAⅢ-Producing) gene from Arthrobacterglobiformis C11-1[J].Journal of Bioscience and Bioengineering, 2000, 89:590-595.
    [8] Cereghino JL, Cregg JM.Heterologous protein expression in the methylotrophic yeast Pichia pastoris[J].FEMS Microbiology Reviews, 2000, 24:45-66.
    [9] Fan YH, Zhang YJ, Yang XY, et al.Expression of a Bearveria bassiana chitinase (Bbchit1) in Escherichia coli and Pichia pastoris[J].Pretein Expression and Purification, 2007, 56:93-99.
    [10] Jahnz U, Schubert M, Baars-Hibbe H, et al.Process for producing the potential food ingredient DFAⅢfrom inulin:screening, genetic engineering, fermentation and immobilisation of inulase II[J].International Journal of Pharmaceutics, 2003, 256:199-20.
    [11] Haraguchi K, Yoshida M, Ohtsubo K.Thermostable inulin fructotransferase (DFAⅢ-producing) from Arthrobacter sp.L68-1[J].Carbohydrate Polymers, 2005, 59 (4) :411-416.
    [12] Gellissen G.Heterologous protein production in methylotrophic yeasts[J].Appl Microbiol Biotechnol, 2000, 54:741-750.
    [13] 樊英, 李天保, 杨秀生, 等.巴斯德毕赤酵母表达外源蛋白的优化策略[J].生物技术通报, 2010, 12:41-45.
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  • 收稿日期:  2012-06-11

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